Overview

  • Product name
  • Description
    Rabbit polyclonal to ATG2B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 117-146 of Human ATG2B (NP_060506.5).

  • Positive control
    • CEM cell line lysates. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MCF-7.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab116215 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab116215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 233 kDa.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Sequence similarities
    Belongs to the ATG2 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2410024A21Rik antibody
    • AI047755 antibody
    • AI503411 antibody
    • ATG2 autophagy related 2 homolog B antibody
    • Atg2b antibody
    • ATG2B_HUMAN antibody
    • Autophagy-related protein 2 homolog B antibody
    • AW558123 antibody
    • C030004M05Rik antibody
    • C14orf103 antibody
    • C630028L02Rik antibody
    • FLJ10242 antibody
    • KIAA4067 antibody
    • mKIAA4067 antibody
    see all

Images

  • Anti-ATG2B antibody (ab116215) at 1/100 dilution + CEM cell line lysate at 35 µg

    Predicted band size: 233 kDa

  • ab116215 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab116215 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab116215 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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