For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 117-146 of Human ATG2B (NP_060506.5).
Our Abpromise guarantee covers the use of ab116215 in the following tested applications.
|WB||1/100 - 1/500. Predicted molecular weight: 233 kDa.|
|ICC/IF||Use a concentration of 5 µg/ml.|
ab116215 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab116215 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab116215 has not yet been referenced specifically in any publications.