Overview

  • Product nameAnti-ATG4A antibody [EPR4122]
    See all ATG4A primary antibodies
  • Description
    Rabbit monoclonal [EPR4122] to ATG4A
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human ATG4A

  • Positive control
    • Fetal liver, HepG2, Jurkat and fetal brain lysates. Human brain tissue and Human skeletal muscle tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Applications

Our Abpromise guarantee covers the use of ab108322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform antigen retrieval.
  • Application notesIs unsuitable for Flow Cyt or ICC.
  • Target

    • FunctionCysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP.
    • Tissue specificityWidely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver.
    • Sequence similaritiesBelongs to the peptidase C54 family.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • AI627006 antibody
      • Apg4a antibody
      • ATG4 autophagy related 4 homolog A (S. cerevisiae) antibody
      • ATG4A antibody
      • ATG4A_HUMAN antibody
      • Atg4al antibody
      • AUT like 2 cysteine endopeptidase antibody
      • AUT-like 2 cysteine endopeptidase antibody
      • Autl2 antibody
      • Autophagin 2 antibody
      • Autophagin-2 antibody
      • Autophagy related 4A cysteine peptidase antibody
      • Autophagy related cysteine endopeptidase 2 antibody
      • Autophagy related protein 4 homolog A antibody
      • Autophagy-related cysteine endopeptidase 2 antibody
      • Autophagy-related protein 4 homolog A antibody
      • AV169859 antibody
      • Cysteine protease ATG4A antibody
      • hAPG4A antibody
      • MGC107179 antibody
      see all

    Anti-ATG4A antibody [EPR4122] images

    • All lanes : Anti-ATG4A antibody [EPR4122] (ab108322) at 1/1000 dilution

      Lane 1 : Fetal liver lysate
      Lane 2 : HepG2 lysate
      Lane 3 : Jurkat lysate
      Lane 4 : Fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 45 kDa
    • Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab108322 at 1/100 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue using ab108322 at 1/100 dilution.

    References for Anti-ATG4A antibody [EPR4122] (ab108322)

    ab108322 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"