The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
Application notesIs unsuitable for WB.
FunctionCysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
Tissue specificityMainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.
Sequence similaritiesBelongs to the peptidase C54 family.
Detection of ATG4B by Western Blot of Immunprecipitate.
An antibody to ATG4B, at 1 µg/ml, staining ATG4B in HeLa whole cell lysate immunoprecipitated using ab101983 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 30 seconds.
References for Anti-ATG4B antibody (ab101983)
has not yet been referenced specifically in any publications.
Publishing research using ab101983? Please let us know so that we can cite the reference in this datasheet.
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