Anti-ATG4B antibody (ab69937)

Overview

  • Product nameAnti-ATG4B antibody
    See all ATG4B primary antibodies
  • Description
    Rabbit polyclonal to ATG4B
  • SpecificityDetects endogenous levels of total ATG4B protein.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from internal of human ATG4B.

  • Positive control
    • Human skeletal muscle tissue Jurkat cell extract

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab69937 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
ELISA 1/20000.
IHC-P 1/50 - 1/100.

Target

  • FunctionCysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
  • Tissue specificityMainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.
  • Sequence similaritiesBelongs to the peptidase C54 family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • APG4 autophagy 4 homolog B antibody
    • APG4B antibody
    • ATG4 autophagy related 4 homolog B (S. cerevisiae) antibody
    • ATG4B antibody
    • ATG4B_HUMAN antibody
    • AUT like 1 cysteine endopeptidase antibody
    • AUT-like 1 cysteine endopeptidase antibody
    • AUTL1 antibody
    • Autophagin 1 antibody
    • Autophagin-1 antibody
    • Autophagy related cysteine endopeptidase 1 antibody
    • Autophagy related protein 4 homolog B antibody
    • Autophagy-related cysteine endopeptidase 1 antibody
    • Autophagy-related protein 4 homolog B antibody
    • Cysteine protease ATG4B antibody
    • hAPG4B antibody
    • MGC1353 antibody
    see all

Anti-ATG4B antibody images

  • Immunohistochemical analysis of paraffin embedded human skeletal muscle tissue labelled with ab69937 at 1/50-1/100 dilution. Right panel was also treated with immunising peptide.
  • All lanes : Anti-ATG4B antibody (ab69937) at 1/500 dilution

    Lane 1 : Jurkat cell extract
    Lane 2 : Jurkat cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 44 kDa
    Observed band size : 44 kDa
    Additional bands at : 34 kDa,70 kDa. We are unsure as to the identity of these extra bands.

References for Anti-ATG4B antibody (ab69937)

ab69937 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam. I have found out about the immunogen sequences for two of the antibodies that your are interested in. These are ab70876 and ab69937. For ab70876 the the immunogen sequence is from AA** of human ATG4B and for ab69937, the...

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Abreviews
Application Western blot
Sample Mouse Cell lysate - whole cell (P388D1, macrophages)
Loading amount 10 µg
Specification P388D1, macrophages
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Abcam user community

Verified customer

Submitted Sep 10 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (alveolar basal epithelial cells)
Loading amount 10 µg
Specification alveolar basal epithelial cells
Gel Running Conditions Reduced Denaturing (12)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Sep 07 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"