Overview

  • Product nameAnti-ATG4B antibody
    See all ATG4B primary antibodies
  • Description
    Rabbit polyclonal to ATG4B
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 350-393 of Human ATG4B. (NP_037457).

  • Positive control
    • HeLa and RAW cell lysates and human heart tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab72201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 44 kDa).
IHC-P Use a concentration of 10 µg/ml.

Target

  • FunctionCysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
  • Tissue specificityMainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.
  • Sequence similaritiesBelongs to the peptidase C54 family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • APG4 autophagy 4 homolog B antibody
    • APG4B antibody
    • ATG4 autophagy related 4 homolog B (S. cerevisiae) antibody
    • ATG4B antibody
    • ATG4B_HUMAN antibody
    • AUT like 1 cysteine endopeptidase antibody
    • AUT-like 1 cysteine endopeptidase antibody
    • AUTL1 antibody
    • Autophagin 1 antibody
    • Autophagin-1 antibody
    • Autophagy related cysteine endopeptidase 1 antibody
    • Autophagy related protein 4 homolog B antibody
    • Autophagy-related cysteine endopeptidase 1 antibody
    • Autophagy-related protein 4 homolog B antibody
    • Cysteine protease ATG4B antibody
    • hAPG4B antibody
    • MGC1353 antibody
    see all

Anti-ATG4B antibody images

  • ab72201 at 10µg/ml staining ATG4B in human heart by Immunohistochemistry, Paraffin-embedded tissue.
  • All lanes : Anti-ATG4B antibody (ab72201) at 2 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate with immunizing peptide
    Lane 3 : RAW cell lysate


    Predicted band size : 44 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.

References for Anti-ATG4B antibody (ab72201)

ab72201 has not yet been referenced specifically in any publications.

Product Wall

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