Anti-ATG4C antibody - C-terminal (ab191705)
Key features and details
- Rabbit polyclonal to ATG4C - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-ATG4C antibody - C-terminal
See all ATG4C primary antibodies -
Description
Rabbit polyclonal to ATG4C - C-terminal -
Host species
Rabbit -
Specificity
ab191705 is predicted to not cross-react with other ATG4 proteins. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human ATG4C (C terminal). An 18 amino acid peptide near the carboxy terminus of Human ATG4C. NP_835739.
Database link: Q96DT6 -
Positive control
- A549 cell lysate. Human lung tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191705 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 52 kDa).
|
|
IHC-P |
Use a concentration of 5 - 20 µg/ml.
|
Notes |
---|
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 52 kDa). |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
-
Function
Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. -
Tissue specificity
Highly expressed in skeletal muscle, heart, liver and testis. -
Sequence similarities
Belongs to the peptidase C54 family. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 84938 Human
- Omim: 611339 Human
- SwissProt: Q96DT6 Human
- Unigene: 7353 Human
-
Alternative names
- APG4 autophagy 4 homolog C (S. cerevisiae) antibody
- APG4 autophagy 4 homolog C antibody
- APG4 C antibody
see all
Images
-
Anti-ATG4C antibody - C-terminal (ab191705) at 1 µg/ml + A549 cell lysate at 15 µg
Predicted band size: 52 kDa -
Immunofluorescent analysis of human lung tissue labeling ATG4C with ab181391 at 20 µg/ml
-
Immunohistochemical analysis of human lung tissue labeling ATG4C with ab191705 at 5 µg/ml.
Protocols
Datasheets and documents
-
Datasheet download
References (1)
ab191705 has been referenced in 1 publication.
- Liu H et al. Downregulation of DLL4 predicts poor survival in non-small cell lung cancer patients due to promotion of lymph node metastasis. Oncol Rep 40:2988-2996 (2018). PubMed: 30226615