The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
Highly expressed in skeletal muscle, heart, liver and testis.
Flow cytometric analysis of 2% paraformaldehyde-fixed 293 cells labeling ATG4C with ab183516 at 1/100 dilution (green). Goat anti-rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control (red).