• Product nameAnti-ATIC antibody
    See all ATIC primary antibodies
  • Description
    Rabbit polyclonal to ATIC
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Saccharomyces cerevisiae, Drosophila melanogaster, Zebrafish
  • Immunogen

    A region within synthetic peptide: HTAQYDEAIS DYFRKQYSKG VSQMPLRYGM NPHQTPAQLY TLQPKLPITV, corresponding to amino acids 181-230 of Human ATIC

  • Positive control
    • HepG2 cell lysate



Our Abpromise guarantee covers the use of ab69827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 65 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • FunctionBifunctional enzyme that catalyzes 2 steps in purine biosynthesis.
  • PathwayPurine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1.
    Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
  • Involvement in diseaseDefects in ATIC are the cause of AICA-ribosuria [MIM:608688]; also known as AICA-ribosiduria. AICA-ribosuria is a neurologically devastating inborn error of purine biosynthesis. AICA-ribosuria patients excrete massive amounts of AICA-riboside in the urine and accumulate AICA-ribotide and its derivatives in erythrocytes and fibroblasts. AICA-ribosuria causes profound mental retardation, epilepsy, dysmorphic features and congenital blindness.
  • Sequence similaritiesBelongs to the purH family.
  • DomainThe IMP cyclohydrolase activity resides in the N-terminal region.
  • Information by UniProt
  • Database links
  • Alternative names
    • AICAR formyltransferase/IMP cyclohydrolase antibody
    • 5 aminoimidazole 4 carboxamide 1 beta D ribonucleotide transformylase/inosinicase antibody
    • 5 aminoimidazole 4 carboxamide ribonucleotide formyltransferase antibody
    • 5 aminoimidazole 4 carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase antibody
    • 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase antibody
    • AICAR antibody
    • AICAR formyltransferase/IMP cyclohydrolase bifunctional enzyme antibody
    • AICAR transformylase antibody
    • AICARFT antibody
    • AICARFT/IMPCHASE antibody
    • ATIC antibody
    • Bifunctional purine biosynthesis protein PURH antibody
    • epididymis secretory sperm binding protein Li 70p antibody
    • FLJ93545 antibody
    • HEL-S-70p antibody
    • IMP cyclohydrolase antibody
    • IMP synthase antibody
    • IMP synthetase antibody
    • IMPCHASE antibody
    • Inosinicase antibody
    • OK/SW-cl.86 antibody
    • Phosphoribosylaminoimidazolecarboxamide formyltransferase antibody
    • Phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase antibody
    • PUR9_HUMAN antibody
    • PURH antibody
    see all

Anti-ATIC antibody images

  • Anti-ATIC antibody (ab69827) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 65 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-ATIC antibody (ab69827) at 1/800 dilution

    Lane 1 : Hamster CHO K1 cells at 20 µg
    Lane 2 : HeLa cells at 20 µg
    Lane 3 : HEK273 cells at 20 µg
    Lane 4 : Human Skin Fibroblasts at 100 µg

    Predicted band size : 65 kDa

    Secondary Dilution: 1/2000

References for Anti-ATIC antibody (ab69827)

ab69827 has not yet been referenced specifically in any publications.

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