Recombinant
RabMAb

Anti-ATIC antibody [EPR13243-53] (ab188321)

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Overview

  • Product name
    Anti-ATIC antibody [EPR13243-53]
    See all ATIC primary antibodies
  • Description
    Rabbit monoclonal [EPR13243-53] to ATIC
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human ATIC aa 450 to the C-terminus. The exact sequence is proprietary.
    Database link: P31939

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Jurkat, HeLa, HepG2 and HCT-116 cell lysates; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab188321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
Flow Cyt 1/140.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IP 1/40 - 1/60.

Target

  • Function
    Bifunctional enzyme that catalyzes 2 steps in purine biosynthesis.
  • Pathway
    Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1.
    Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
  • Involvement in disease
    Defects in ATIC are the cause of AICA-ribosuria [MIM:608688]; also known as AICA-ribosiduria. AICA-ribosuria is a neurologically devastating inborn error of purine biosynthesis. AICA-ribosuria patients excrete massive amounts of AICA-riboside in the urine and accumulate AICA-ribotide and its derivatives in erythrocytes and fibroblasts. AICA-ribosuria causes profound mental retardation, epilepsy, dysmorphic features and congenital blindness.
  • Sequence similarities
    Belongs to the purH family.
  • Domain
    The IMP cyclohydrolase activity resides in the N-terminal region.
  • Information by UniProt
  • Database links
    • Alternative names
      • AICAR formyltransferase/IMP cyclohydrolase antibody
      • 5 aminoimidazole 4 carboxamide 1 beta D ribonucleotide transformylase/inosinicase antibody
      • 5 aminoimidazole 4 carboxamide ribonucleotide formyltransferase antibody
      • 5 aminoimidazole 4 carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase antibody
      • 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase antibody
      • AICAR antibody
      • AICAR formyltransferase/IMP cyclohydrolase bifunctional enzyme antibody
      • AICAR transformylase antibody
      • AICARFT antibody
      • AICARFT/IMPCHASE antibody
      • ATIC antibody
      • Bifunctional purine biosynthesis protein PURH antibody
      • epididymis secretory sperm binding protein Li 70p antibody
      • FLJ93545 antibody
      • HEL-S-70p antibody
      • IMP cyclohydrolase antibody
      • IMP synthase antibody
      • IMP synthetase antibody
      • IMPCHASE antibody
      • Inosinicase antibody
      • OK/SW-cl.86 antibody
      • Phosphoribosylaminoimidazolecarboxamide formyltransferase antibody
      • Phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase antibody
      • PUR9_HUMAN antibody
      • PURH antibody
      see all

    Images

    • Western blot - Anti-ATIC [EPR13243-53] antibody (ab188321)
      Western blot - Anti-ATIC [EPR13243-53] antibody (ab188321)
      All lanes : Anti-ATIC antibody [EPR13243-53] (ab188321) at 1/10000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : HCT-116 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 65 kDa
      Observed band size: 65 kDa

    • Flow Cytometry - Anti-ATIC [EPR13243-53] antibody (ab188321)
      Flow Cytometry - Anti-ATIC [EPR13243-53] antibody (ab188321)

      Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling ATIC with ab188321 at 1/140 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

    • Immunoprecipitation - Anti-ATIC [EPR13243-53] antibody (ab188321)
      Immunoprecipitation - Anti-ATIC [EPR13243-53] antibody (ab188321)

      Western blot analysis of ATIC in Jurkat cell lysate immunoprecipitated with ab188321 at 1/50 dilution (Lane 1). Lane 2: PBS instead of Jurkat lysate.

      Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    References

    ab188321 has not yet been referenced specifically in any publications.

    Publishing research using ab188321? Please let us know so that we can cite the reference in this datasheet.

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