Recombinant
RabMAb

Anti-ATIC antibody [EPR13243-53] (ab188321)

Overview

  • Product name
    Anti-ATIC antibody [EPR13243-53]
    See all ATIC primary antibodies
  • Description
    Rabbit monoclonal [EPR13243-53] to ATIC
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human ATIC aa 450 to the C-terminus. The exact sequence is proprietary.
    Database link: P31939

  • Positive control
    • Jurkat, HeLa, HepG2 and HCT-116 cell lysates; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab188321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
Flow Cyt 1/140.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/40 - 1/60.

Target

  • Function
    Bifunctional enzyme that catalyzes 2 steps in purine biosynthesis.
  • Pathway
    Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1.
    Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
  • Involvement in disease
    Defects in ATIC are the cause of AICA-ribosuria [MIM:608688]; also known as AICA-ribosiduria. AICA-ribosuria is a neurologically devastating inborn error of purine biosynthesis. AICA-ribosuria patients excrete massive amounts of AICA-riboside in the urine and accumulate AICA-ribotide and its derivatives in erythrocytes and fibroblasts. AICA-ribosuria causes profound mental retardation, epilepsy, dysmorphic features and congenital blindness.
  • Sequence similarities
    Belongs to the purH family.
  • Domain
    The IMP cyclohydrolase activity resides in the N-terminal region.
  • Information by UniProt
  • Database links
  • Alternative names
    • AICAR formyltransferase/IMP cyclohydrolase antibody
    • 5 aminoimidazole 4 carboxamide 1 beta D ribonucleotide transformylase/inosinicase antibody
    • 5 aminoimidazole 4 carboxamide ribonucleotide formyltransferase antibody
    • 5 aminoimidazole 4 carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase antibody
    • 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase antibody
    • AICAR antibody
    • AICAR formyltransferase/IMP cyclohydrolase bifunctional enzyme antibody
    • AICAR transformylase antibody
    • AICARFT antibody
    • AICARFT/IMPCHASE antibody
    • ATIC antibody
    • Bifunctional purine biosynthesis protein PURH antibody
    • epididymis secretory sperm binding protein Li 70p antibody
    • FLJ93545 antibody
    • HEL-S-70p antibody
    • IMP cyclohydrolase antibody
    • IMP synthase antibody
    • IMP synthetase antibody
    • IMPCHASE antibody
    • Inosinicase antibody
    • OK/SW-cl.86 antibody
    • Phosphoribosylaminoimidazolecarboxamide formyltransferase antibody
    • Phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase antibody
    • PUR9_HUMAN antibody
    • PURH antibody
    see all

Images

  • All lanes : Anti-ATIC antibody [EPR13243-53] (ab188321) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : HCT-116 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 65 kDa
    Observed band size: 65 kDa

  • Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling ATIC with ab188321 at 1/140 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Western blot analysis of ATIC in Jurkat cell lysate immunoprecipitated with ab188321 at 1/50 dilution (Lane 1). Lane 2: PBS instead of Jurkat lysate.

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

References

ab188321 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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