Overview

  • Product nameAnti-ATIC antibody [F38 P7 H9]
    See all ATIC primary antibodies
  • Description
    Mouse monoclonal [F38 P7 H9] to ATIC
  • SpecificityThis antibody is specific to ATIC.
  • Tested applicationsSuitable for: IHC-P, Flow Cyt, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Xenopus laevis, Drosophila melanogaster
    Predicted to work with: Chicken
  • Immunogen

    Synthetic peptide corresponding to Human ATIC aa 583-592 conjugated to Ovalbumin.
    Sequence:

    AHTNLRLFHH

  • Positive control
    • colon carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab33520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 22180458

Target

  • FunctionBifunctional enzyme that catalyzes 2 steps in purine biosynthesis.
  • PathwayPurine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1.
    Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
  • Involvement in diseaseDefects in ATIC are the cause of AICA-ribosuria [MIM:608688]; also known as AICA-ribosiduria. AICA-ribosuria is a neurologically devastating inborn error of purine biosynthesis. AICA-ribosuria patients excrete massive amounts of AICA-riboside in the urine and accumulate AICA-ribotide and its derivatives in erythrocytes and fibroblasts. AICA-ribosuria causes profound mental retardation, epilepsy, dysmorphic features and congenital blindness.
  • Sequence similaritiesBelongs to the purH family.
  • DomainThe IMP cyclohydrolase activity resides in the N-terminal region.
  • Information by UniProt
  • Database links
  • Alternative names
    • AICAR formyltransferase/IMP cyclohydrolase antibody
    • 5 aminoimidazole 4 carboxamide 1 beta D ribonucleotide transformylase/inosinicase antibody
    • 5 aminoimidazole 4 carboxamide ribonucleotide formyltransferase antibody
    • 5 aminoimidazole 4 carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase antibody
    • 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase antibody
    • AICAR antibody
    • AICAR formyltransferase/IMP cyclohydrolase bifunctional enzyme antibody
    • AICAR transformylase antibody
    • AICARFT antibody
    • AICARFT/IMPCHASE antibody
    • ATIC antibody
    • Bifunctional purine biosynthesis protein PURH antibody
    • epididymis secretory sperm binding protein Li 70p antibody
    • FLJ93545 antibody
    • HEL-S-70p antibody
    • IMP cyclohydrolase antibody
    • IMP synthase antibody
    • IMP synthetase antibody
    • IMPCHASE antibody
    • Inosinicase antibody
    • OK/SW-cl.86 antibody
    • Phosphoribosylaminoimidazolecarboxamide formyltransferase antibody
    • Phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase antibody
    • PUR9_HUMAN antibody
    • PURH antibody
    see all

Anti-ATIC antibody [F38 P7 H9] images

  • ab33520, at a concentration of 5 µg/ml, staining ATIC in Human colon carcinoma tissue by Immunohistochemistry.

  • Overlay histogram showing HeLa cells stained with ab33520 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab33520, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-ATIC antibody [F38 P7 H9] (ab33520)

This product has been referenced in:
  • Parviainen VI  et al. Label-free mass spectrometry proteome quantification of human embryonic kidney cells following 24 hours of sialic acid overproduction. Proteome Sci 11:38 (2013). WB . Read more (PubMed: 23915316) »
  • Baresova V  et al. Mutations of ATIC and ADSL affect purinosome assembly in cultured skin fibroblasts from patients with AICA-ribosiduria and ADSL deficiency. Hum Mol Genet 21:1534-43 (2012). ICC/IF ; Human . Read more (PubMed: 22180458) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (head/neck tumor)
Specification head/neck tumor
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH6.0
Permeabilization No
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 1.5%
Username

Abcam user community

Verified customer

Submitted Jan 02 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"