Overview

  • Product nameAnti-ATL3 antibody
    See all ATL3 primary antibodies
  • Description
    Rabbit polyclonal to ATL3
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Guinea pig, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 350-400 of Human ATL3 according to NP_056274.3.

  • Positive control
    • Whole cell lysate from HeLa, Jurkat, 293T and mouse NIH3T3 cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab117819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 61 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Anti-ATL3 antibody images

  • All lanes : Anti-ATL3 antibody (ab117819) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : Jurkat whole cell lysate at 50 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 61 kDa


    Exposure time : 30 seconds
  • Detection of Human ATL3 by Western Blot of Immunoprecipitation.
    ab117819 at 1µg/ml staining ATL3 in HeLa whole cell lysate immunoprecipitated using ab117819 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.

References for Anti-ATL3 antibody (ab117819)

ab117819 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Gel Running Conditions Reduced Denaturing (4-12% NuPage gel)
Loading amount 20 µg
Specification HeLa cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Michiel Krols

Verified customer

Submitted Jun 07 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"