Overview

  • Product name
  • Description
    Rabbit polyclonal to ATL3
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 491-541 of Human ATL3 (NP_056274.3).

  • Positive control
    • HeLa, Jurkat, 293T and Mouse NIH3T3 whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 99% Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab117834 was affinity purified using an epitope specific to ATL3 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab117834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 61 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    GTPase tethering membranes through formation of trans-homooligomer and mediating homotypic fusion of endoplasmic reticulum membranes. Functions in endoplasmic reticulum tubular network biogenesis.
  • Tissue specificity
    Expressed in peripheral tissues (at protein level).
  • Sequence similarities
    Belongs to the GBP family. Atlastin subfamily.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4633402C03Rik antibody
    • 5730596K20Rik antibody
    • AI465397 antibody
    • ATL3 antibody
    • ATLA3_HUMAN antibody
    • Atlastin 3 like antibody
    • Atlastin GTPase 3 antibody
    • Atlastin-3 antibody
    • AW228836 antibody
    • DKFZp564J0863 antibody
    • MGC28761 antibody
    see all

Images

  • All lanes : Anti-ATL3 antibody (ab117834) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : Jurkat whole cell lysate at 50 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 61 kDa


    Exposure time : 30 seconds
  • Detection of Human ATL3 by Immunoprecipitation in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab117834 at 6 µg/mg of lysate. Subsequent WB detection was done using 117834 at 1 µg/ml. ECL detection. Exposure time: 10 seconds.

References

ab117834 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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