Overview

  • Product nameAnti-ATM antibody [MAT3-4G10/8]See all ATM primary antibodies ...
  • Description
    Mouse monoclonal [MAT3-4G10/8] to ATM
  • SpecificityThis antibody recognizes ATM.
  • Tested applicationsIndirect ELISA, IP, WB more details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide: YSDKKSTDEQEKRSPTFEEGSQ conjugated to KLH by a Cysteine residue linker at N-terminus, corresponding to amino acids 1967-1988 of Mouse ATM.

  • Positive control
    • Total extract of HEK293T cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab59541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Indirect ELISA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
WB Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 300 kDa (predicted molecular weight: 350 kDa).

Target

  • FunctionSerine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends.
  • Tissue specificityFound in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes.
  • Involvement in diseaseDefects in ATM are the cause of ataxia telangiectasia (AT) [MIM:208900]; also known as Louis-Bar syndrome, which includes four complementation groups: A, C, D and E. This rare recessive disorder is characterized by progressive cerebellar ataxia, dilation of the blood vessels in the conjunctiva and eyeballs, immunodeficiency, growth retardation and sexual immaturity. AT patients have a strong predisposition to cancer; about 30% of patients develop tumors, particularly lymphomas and leukemias. Cells from affected individuals are highly sensitive to damage by ionizing radiation and resistant to inhibition of DNA synthesis following irradiation.
    Note=Defects in ATM contribute to T-cell acute lymphoblastic leukemia (TALL) and T-prolymphocytic leukemia (TPLL). TPLL is characterized by a high white blood cell count, with a predominance of prolymphocytes, marked splenomegaly, lymphadenopathy, skin lesions and serous effusion. The clinical course is highly aggressive, with poor response to chemotherapy and short survival time. TPLL occurs both in adults as a sporadic disease and in younger AT patients.
    Note=Defects in ATM contribute to B-cell non-Hodgkin lymphomas (BNHL), including mantle cell lymphoma (MCL).
    Note=Defects in ATM contribute to B-cell chronic lymphocytic leukemia (BCLL). BCLL is the commonest form of leukemia in the elderly. It is characterized by the accumulation of mature CD5+ B lymphocytes, lymphadenopathy, immunodeficiency and bone marrow failure.
  • Sequence similaritiesBelongs to the PI3/PI4-kinase family. ATM subfamily.
    Contains 1 FAT domain.
    Contains 1 FATC domain.
    Contains 1 PI3K/PI4K domain.
  • DomainThe FATC domain is required for interaction with KAT5.
  • Post-translational
    modifications
    Phosphorylated by NUAK1/ARK5. Autophosphorylation on Ser-367, Ser-1893, Ser-1981 correlates with DNA damage-mediated activation of the kinase.
    Acetylation, on DNA damage, is required for activation of the kinase activity, dimer-monomer transition, and subsequent autophosphorylation on Ser-1981. Acetylated in vitro by KAT5/TIP60.
  • Cellular localizationNucleus. Cytoplasmic vesicle. Primarily nuclear. Found also in endocytic vesicles in association with beta-adaptin.
  • Target information above from: UniProt accession Q13315 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • A-T mutated antibody
    • A-T mutated homolog antibody
    • AT complementation group A antibody
    • AT complementation group C antibody
    • AT complementation group D antibody
    • AT complementation group E antibody
    • AT mutated antibody
    • AT protein antibody
    • AT1 antibody
    • ATA antibody
    • Ataxia telangiectasia gene mutated in human beings antibody
    • Ataxia telangiectasia mutated antibody
    • Ataxia telangiectasia mutated gene antibody
    • Ataxia telangiectasia mutated homolog (human) antibody
    • Ataxia telangiectasia mutated homolog antibody
    • ATC antibody
    • ATD antibody
    • ATDC antibody
    • ATE antibody
    • ATM antibody
    • ATM_HUMAN antibody
    • DKFZp781A0353 antibody
    • Human phosphatidylinositol 3 kinase homolog antibody
    • MGC74674 antibody
    • OTTHUMP00000232981 antibody
    • Serine protein kinase ATM antibody
    • Serine-protein kinase ATM antibody
    • Serine/threonine-protein kinase ATM antibody
    • T cell prolymphocytic leukemia antibody
    • Tefu antibody
    • TEL1 antibody
    • TEL1, telomere maintenance 1, homolog antibody
    • TELO1 antibody
    • Telomere fusion protein antibody
    • TPLL antibody
    see all

Anti-ATM antibody [MAT3-4G10/8] images

  • Lane 1 : Anti-ATM antibody [MAT3-4G10/8] (ab59541) at 0.5 µg/ml
    Lane 2 : Anti-ATM antibody [MAT3-4G10/8] (ab59541) at 0.25 µg/ml
    Lane 3 : Anti-ATM antibody [MAT3-4G10/8] (ab59541) at 0.125 µg/ml

    Lane 1 : Total cell extract of HEK-293T cells
    Lane 2 : Total cell extract of HEK-293T cells
    Lane 3 : Total cell extract of HEK-293T cells

    Secondary
    Goat Anti-Mouse, Peroxidase conjugate

    Predicted band size : 350 kDa
    Observed band size : 300 kDa (why is the actual band size different from the predicted?)

References for Anti-ATM antibody [MAT3-4G10/8] (ab59541)

This product has been referenced in:
  • Parsons JL  et al. Phosphorylation of PNKP by ATM prevents its proteasomal degradation and enhances resistance to oxidative stress. Nucleic Acids Res : (2012). WB ; Human . Read more (PubMed: 23042680) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 30 µg
Specification HeLa cells
Treatment ATM specific siRNA for 48h
Gel Running Conditions Reduced Denaturing (4-20% Tris-Gly gel)
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
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Abcam user community

Verified customer

Submitted May 28 2012

Thank you for your call and for your patience while I have looked into your enquiry.

I have been in touch with the lab, and this antibody is not tested in-house in immunoprecipitation, so unfortunately we don't have an optimized protocol. Thi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"