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Synthetic peptide (Human) with a phosphorylated serine (1981)
Our Abpromise guarantee covers the use of ab36810 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/1000. Detects a band of approximately 370 kDa (predicted molecular weight: 370 kDa).
Abcam recommends using 3% Milk as the blocking agent.
|IHC-P||Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab36810 staining ATM (phospho S1981) in T98G Human brain glioblastoma cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, pH 7.4 for 5 minutes at room temperature and blocked with 5% BSA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/250 in PBS) for 1 hour. A CF488A-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.
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