Overview

  • Product nameAnti-ATM [Y170] antibodySee all ATM primary antibodies ...
  • Description
    Rabbit monoclonal [Y170] to ATM
  • SpecificityThis antibody is specific for ATM.
  • Tested applicationsWB, IHC-P, ICC/IF, IP more details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to residues surrounding Serine 1981 of human ATM.

  • Positive control
    • WB: 293 cell lysate. IHC-P: Human breast carcinoma.
  • General notes

    Produced under U.S. Patent No. 5,675,063.

    This product is available conjugated to DyLight® 488 see ab139830.

    A 40 μl trial size is available to purchase for this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
IHC-P
ICC/IF
IP
  • Application notesICC/IF: 1/100 - 1/250.
    IHC-P: 1/100 - 1/250.
    IP: 1/50.
    WB: 1/5000. Predicted molecular weight: 350 kDa.

    Is unsuitable for FACS.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionSerine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends.
    • Tissue specificityFound in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes.
    • Involvement in diseaseDefects in ATM are the cause of ataxia telangiectasia (AT) [MIM:208900]; also known as Louis-Bar syndrome, which includes four complementation groups: A, C, D and E. This rare recessive disorder is characterized by progressive cerebellar ataxia, dilation of the blood vessels in the conjunctiva and eyeballs, immunodeficiency, growth retardation and sexual immaturity. AT patients have a strong predisposition to cancer; about 30% of patients develop tumors, particularly lymphomas and leukemias. Cells from affected individuals are highly sensitive to damage by ionizing radiation and resistant to inhibition of DNA synthesis following irradiation.
      Note=Defects in ATM contribute to T-cell acute lymphoblastic leukemia (TALL) and T-prolymphocytic leukemia (TPLL). TPLL is characterized by a high white blood cell count, with a predominance of prolymphocytes, marked splenomegaly, lymphadenopathy, skin lesions and serous effusion. The clinical course is highly aggressive, with poor response to chemotherapy and short survival time. TPLL occurs both in adults as a sporadic disease and in younger AT patients.
      Note=Defects in ATM contribute to B-cell non-Hodgkin lymphomas (BNHL), including mantle cell lymphoma (MCL).
      Note=Defects in ATM contribute to B-cell chronic lymphocytic leukemia (BCLL). BCLL is the commonest form of leukemia in the elderly. It is characterized by the accumulation of mature CD5+ B lymphocytes, lymphadenopathy, immunodeficiency and bone marrow failure.
    • Sequence similaritiesBelongs to the PI3/PI4-kinase family. ATM subfamily.
      Contains 1 FAT domain.
      Contains 1 FATC domain.
      Contains 1 PI3K/PI4K domain.
    • DomainThe FATC domain is required for interaction with KAT5.
    • Post-translational
      modifications
      Phosphorylated by NUAK1/ARK5. Autophosphorylation on Ser-367, Ser-1893, Ser-1981 correlates with DNA damage-mediated activation of the kinase.
      Acetylation, on DNA damage, is required for activation of the kinase activity, dimer-monomer transition, and subsequent autophosphorylation on Ser-1981. Acetylated in vitro by KAT5/TIP60.
    • Cellular localizationNucleus. Cytoplasmic vesicle. Primarily nuclear. Found also in endocytic vesicles in association with beta-adaptin.
    • Target information above from: UniProt accession Q13315 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • A-T mutated antibody
      • A-T mutated homolog antibody
      • AT complementation group A antibody
      • AT complementation group C antibody
      • AT complementation group D antibody
      • AT complementation group E antibody
      • AT mutated antibody
      • AT protein antibody
      • AT1 antibody
      • ATA antibody
      • Ataxia telangiectasia gene mutated in human beings antibody
      • Ataxia telangiectasia mutated antibody
      • Ataxia telangiectasia mutated gene antibody
      • Ataxia telangiectasia mutated homolog (human) antibody
      • Ataxia telangiectasia mutated homolog antibody
      • ATC antibody
      • ATD antibody
      • ATDC antibody
      • ATE antibody
      • ATM antibody
      • ATM_HUMAN antibody
      • DKFZp781A0353 antibody
      • Human phosphatidylinositol 3 kinase homolog antibody
      • MGC74674 antibody
      • OTTHUMP00000232981 antibody
      • Serine protein kinase ATM antibody
      • Serine-protein kinase ATM antibody
      • Serine/threonine-protein kinase ATM antibody
      • T cell prolymphocytic leukemia antibody
      • Tefu antibody
      • TEL1 antibody
      • TEL1, telomere maintenance 1, homolog antibody
      • TELO1 antibody
      • Telomere fusion protein antibody
      • TPLL antibody
      see all

    Anti-ATM [Y170] antibody images

    • ab32420, at a 1/100 dilution, staining ATM in 293 cells by Immunofluorescence.

    • Anti-ATM [Y170] antibody (ab32420) at 1/5000 dilution + 293 cell lysate.

      Predicted band size : 350 kDa
    • ab32420, at a 1/100 dilution, staining ATM in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.

      Ab32420, at a 1/100 dilution, staining ATM in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
    •  ab32420 showing positive staining in Normal tonsil tissue.

    • ab32420 showing positive staining in Endometrial carcinoma tissue.

    •  ab32420 showing positive staining in Glioma tissue.

    • ab32420 showing positive staining in Breast carcinoma tissue.

    • ab32420 showing positive staining in Urinary bladder transitional carcinoma tissue.

    References for Anti-ATM [Y170] antibody (ab32420)

    This product has been referenced in:
    • Lundholm L  et al. Resistance to DNA-damaging treatment in non-small cell lung cancer tumor-initiating cells involves reduced DNA-PK/ATM activation and diminished cell cycle arrest. Cell Death Dis 4:e478 (2013). WB ; Human . Read more (PubMed: 23370278) »
    • Zhang ZZ  et al. Loss of BRCA1 expression leads to worse survival in patients with gastric carcinoma. World J Gastroenterol 19:1968-74 (2013). IHC-P ; Human . Read more (PubMed: 23569343) »

    See all 17 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 20% · Temperature: RT°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA Buffer
    Sample Human Tissue sections (Serous Ovarian Tumour)
    Specification Serous Ovarian Tumour
    Permeabilization No
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jul 15 2013

    The blocking peptide for the Anti-ATM antibody [Y170] (ab32420) has now been added to the catalogue following your enquiry. This can be accessed under the catalogue number ab170988


    For producing the IHC-P images, including the glioma tissue, on the datasheet for ab32420, we used the following protocol with a sodium citrate heat mediated antigen retrieval:
    http://www.epitomics.com/products/support/ihc
    We had success w...

    Read More

    Gracias por tu respuesta.
    Efectivamente, si haces un pedido de ab26, el ab32420 os saldría gratuito, al entrar en la promoción de RabMabs.
    Simplemente indica el código promocional RABMAB-XBSMG en la casilla destinada para ello cuando realizas...

    Read More
    Application Western blot
    Sample Human Cell lysate - whole cell (HCT116 colon cancer cell line)
    Loading amount 60 µg
    Specification HCT116 colon cancer cell line
    Gel Running Conditions Reduced Denaturing (7%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Mr. Christian Marx

    Verified customer

    Submitted Oct 29 2012

    Thank you for contacting us.
    Unfortunately I cannot find this particular lot number for this product. I have checked the orders from your Organisation for this antibody and found an order from December 2011. The lot number for the vial sent is XXX...

    Read More

    Thank you for contacting us and your interest in our products.

    Epitomics did offer isotype controls specific for different lots of antibody purchased. This was providing the antibody at the same concentration as the antibody to be tested. We d...

    Read More

    Thank you for contacting Abcam.

    The peptides for both ab32420 and ab81292 maps between amino acids ***********of human ATM (UniProt: Q13315).

    Please let me know if there is anything else I can help you with.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"