• Product name
  • Description
    Mouse monoclonal to ATOX1
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: MPKHEFSVDM TCGGCAEAVS RVLNKLGGVK YDIDLPNKKV CIESEHSMDT LLATLKKTGK TVSYLGLE, corresponding to amino acids 1-69 of Human ATOX1

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.



Our Abpromise guarantee covers the use of ab54865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 7 kDa.
IHC-P Use at an assay dependent concentration. PubMed: 20543960
ICC/IF Use a concentration of 10 µg/ml.


  • Function
    Could bind and deliver cytosolic copper to the copper ATPase proteins. May be important in cellular antioxidant defense.
  • Tissue specificity
  • Sequence similarities
    Belongs to the ATX1 family.
    Contains 1 HMA domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATOX1 antibody
    • ATOX1_HUMAN antibody
    • ATX1 antibody
    • ATX1 antioxidant protein 1 homolog (yeast) antibody
    • ATX1 antioxidant protein 1 homolog antibody
    • Copper transport protein antibody
    • Copper transport protein ATOX1 antibody
    • HAH1 antibody
    • Metal transport protein antibody
    • Metal transport protein ATX1 antibody
    • MGC138453 antibody
    • MGC138455 antibody
    see all


  • Predicted band size : 7 kDa
    ATOX1 antibody (ab54865) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab54865 at 10 ug/ml staining ATOX1 in human Hela cells by Immunocytochemistry / Immunofluorescence.


This product has been referenced in:

See all 2 Publications for this product

Customer reviews and Q&As

I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

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I would suggest running a no primary (only secondary antibody) control and dilutingthe secondary antibodyfurther. If it is possible try to use BSA as blocking - instead of milk.

I hope this will help. If...

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Thank you for your enquiry regarding ab54865 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

It seems the antibody recognizes the target at th...

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