Overview

  • Product nameAnti-ATOX1 antibody
    See all ATOX1 primary antibodies
  • Description
    Mouse monoclonal to ATOX1
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: MPKHEFSVDM TCGGCAEAVS RVLNKLGGVK YDIDLPNKKV CIESEHSMDT LLATLKKTGK TVSYLGLE, corresponding to amino acids 1-69 of Human ATOX1

Properties

Applications

Our Abpromise guarantee covers the use of ab54865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 7 kDa.
IHC-P Use at an assay dependent concentration. PubMed: 20543960
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionCould bind and deliver cytosolic copper to the copper ATPase proteins. May be important in cellular antioxidant defense.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the ATX1 family.
    Contains 1 HMA domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATOX1 antibody
    • ATOX1_HUMAN antibody
    • ATX1 antibody
    • ATX1 antioxidant protein 1 homolog (yeast) antibody
    • ATX1 antioxidant protein 1 homolog antibody
    • Copper transport protein antibody
    • Copper transport protein ATOX1 antibody
    • HAH1 antibody
    • Metal transport protein antibody
    • Metal transport protein ATX1 antibody
    • MGC138453 antibody
    • MGC138455 antibody
    see all

Anti-ATOX1 antibody images



  • Predicted band size : 7 kDa
    ATOX1 antibody (ab54865) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab54865 at 10 ug/ml staining ATOX1 in human Hela cells by Immunocytochemistry / Immunofluorescence.

References for Anti-ATOX1 antibody (ab54865)

This product has been referenced in:

See all 2 Publications for this product

Product Wall

I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

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Thank you for your response.

I would suggest running a no primary (only secondary antibody) control and dilutingthe secondary antibodyfurther. If it is possible try to use BSA as blocking - instead of milk.

I hope this will help. If...

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Thank you for your enquiry regarding ab54865 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

It seems the antibody recognizes the target at th...

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Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

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