Recombinant
RabMAb

Anti-ATOX1 antibody [EPR10352] (ab154179)

Overview

  • Product name
    Anti-ATOX1 antibody [EPR10352]
    See all ATOX1 primary antibodies
  • Description
    Rabbit monoclonal [EPR10352] to ATOX1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to amino acids of Human ATOX1 (UniProt ID: O00244).

  • Positive control
    • HepG2, HeLa and 293T cell lysates, Human prostatic hyperplasia tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 7 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/100 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function
      Could bind and deliver cytosolic copper to the copper ATPase proteins. May be important in cellular antioxidant defense.
    • Tissue specificity
      Ubiquitous.
    • Sequence similarities
      Belongs to the ATX1 family.
      Contains 1 HMA domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATOX1 antibody
      • ATOX1_HUMAN antibody
      • ATX1 antibody
      • ATX1 antioxidant protein 1 homolog (yeast) antibody
      • ATX1 antioxidant protein 1 homolog antibody
      • Copper transport protein antibody
      • Copper transport protein ATOX1 antibody
      • HAH1 antibody
      • Metal transport protein antibody
      • Metal transport protein ATX1 antibody
      • MGC138453 antibody
      • MGC138455 antibody
      see all

    Images

    • All lanes : Anti-ATOX1 antibody [EPR10352] (ab154179) at 1/1000 dilution

      Lane 1 : HepG2 cell lysates
      Lane 2 : HeLa cell lysates
      Lane 3 : 293T cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 7 kDa

    • Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling ATOX1 with ab154179 at 1/100 dilution.

    References

    This product has been referenced in:
    • Blockhuys S & Wittung-Stafshede P Copper chaperone Atox1 plays role in breast cancer cell migration. Biochem Biophys Res Commun 483:301-304 (2017). WB ; Human . Read more (PubMed: 28027931) »
    • Yee EMH  et al. Dextran-Catechin inhibits angiogenesis by disrupting copper homeostasis in endothelial cells. Sci Rep 7:7638 (2017). Read more (PubMed: 28794411) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Primary myoblast derived from mouse satellite cell)
    Gel Running Conditions
    Non-reduced Denaturing (10)
    Loading amount
    30 µg
    Treatment
    Proliferating with 20% FCS and Differentiated by serume starvation with 2% horse serum
    Specification
    Primary myoblast derived from mouse satellite cell
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Dr. Teresita Padilla-Benavides

    Verified customer

    Submitted Jul 29 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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