Anti-ATP-binding cassette sub-family A member 3 antibody [3C9] (ab24751)

Overview

  • Product nameAnti-ATP-binding cassette sub-family A member 3 antibody [3C9]
    See all ATP-binding cassette sub-family A member 3 primary antibodies
  • Description
    Mouse monoclonal [3C9] to ATP-binding cassette sub-family A member 3
  • Specificityab24751 recognises P180 Lamellar Body Protein.
  • Tested applicationsSuitable for: ICC, IHC-P, IHC-Fr, ICC/IF, Electron Microscopy, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Tissue, cells or virus corresponding to Rat ATP-binding cassette sub-family A member 3.

  • Positive control
    • IHC-P: FFPE rat lung tissue sections.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: Ascites
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityMonoclonal
  • Clone number3C9
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Electron Microscopy Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 191 kDa.

Target

  • FunctionPlays an important role in the formation of pulmonary surfactant, probably by transporting lipids such as cholesterol.
  • Tissue specificityHighly expressed in lung, followed by brain, pancreas, skeletal muscle and heart. Weakly expressed in placenta, kidney and liver. Also expressed in medullary thyroid carcinoma cells (MTC) and in C-cell carcinoma.
  • Involvement in diseaseDefects in ABCA3 are the cause of pulmonary surfactant metabolism dysfunction type 3 (SMDP3) [MIM:610921]; also called pulmonary alveolar proteinosis due to ABCA3 deficiency. A rare lung disorder due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress.
  • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCA family.
    Contains 2 ABC transporter domains.
  • DomainMultifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABC 3 antibody
    • ABC C antibody
    • ABC C transporter antibody
    • ABC transporter 3 antibody
    • ABC-C transporter antibody
    • ABC3 antibody
    • ABCA 3 antibody
    • Abca3 antibody
    • ABCA3 protein antibody
    • ABCA3_HUMAN antibody
    • ABCC antibody
    • ATP binding cassette 3 antibody
    • ATP binding cassette sub family A (ABC1) member 3 antibody
    • ATP binding cassette sub family A member 3 antibody
    • ATP binding cassette transporter 3 antibody
    • ATP-binding cassette 3 antibody
    • ATP-binding cassette sub-family A member 3 antibody
    • ATP-binding cassette transporter 3 antibody
    • CED7. C. elegans, homolog of antibody
    • EST111653 antibody
    • LBM 180 antibody
    • LBM180 antibody
    • MGC72201 antibody
    • P180 Lamellar Body Protein antibody
    • SMDP3 antibody
    see all

Anti-ATP-binding cassette sub-family A member 3 antibody [3C9] images

  • IHC image of P180 Lamellar Body Protein staining in rat lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab24751, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-ATP-binding cassette sub-family A member 3 antibody [3C9] (ab24751)

This product has been referenced in:
  • Neuland K  et al. Synaptotagmin-7 links fusion-activated Ca²? entry and fusion pore dilation. J Cell Sci 127:5218-27 (2014). Read more (PubMed: 25344253) »
  • Miklavc P  et al. Actin coating and compression of fused secretory vesicles are essential for surfactant secretion: a role for Rho, formins and myosin II. J Cell Sci : (2012). Read more (PubMed: 22427691) »

See all 6 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (alveolar type II cells)
Permeabilization Yes - 0,2% Saponin
Specification alveolar type II cells
Blocking step Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative Paraformaldehyde
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Verified customer

Submitted Sep 29 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (epithelial cells)
Specification epithelial cells
Blocking step (agent) for 30 minute(s) · Concentration: 1% · Temperature: 23°C
Fixative Formaldehyde
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Submitted Jan 27 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (primary lung epithelia cells)
Permeabilization Yes - Saponin
Specification primary lung epithelia cells
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 1%
Fixative Paraformaldehyde
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Submitted Oct 09 2015

Application Western blot
Sample Rat Cell lysate - whole cell (primary lung epithelia cells)
Gel Running Conditions Reduced Denaturing (4-12)
Loading amount 10 µg
Specification primary lung epithelia cells
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 1%
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Submitted Oct 09 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Primary alveolar type II epithelial cells)
Specification Primary alveolar type II epithelial cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.2 saponin
Blocking step Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C
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Submitted Mar 30 2012

Thank you for your telephone enquiry yesterday. Unfortunately, the epitope for this antibody (ab24751) has not been mapped, so it is not known if it is on the outer membrane or on the intracellular section of the protein. I can suggest trying pe...

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Thank you for your enquiry. The antibody was tested on a 5-15% gradient gel using reducing conditions. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (lung)
Specification lung
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1.5
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Verified customer

Submitted Feb 06 2006

Thank you for your enquiry. This antibody was raised against purified rat lamellar body membranes. We do not know the epitope, as epitope mapping was not done. I hope this information helps, please do not hesitate to contact us if you need any m...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"