Anti-ATP-binding cassette sub-family A member 3 antibody (ab99856)

Overview

  • Product name
    Anti-ATP-binding cassette sub-family A member 3 antibody
    See all ATP-binding cassette sub-family A member 3 primary antibodies
  • Description
    Rabbit polyclonal to ATP-binding cassette sub-family A member 3
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human ATP-binding cassette sub-family A member 3 aa 38-209.
    Database link: BC062779.1

  • Positive control
    • WB: Human fetal kidney and fetal lung tissue lysates and Hela cell lysate. ICC/IF: T47D

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/1000 - 1/2000. Predicted molecular weight: 191 kDa.

Target

  • Function
    Plays an important role in the formation of pulmonary surfactant, probably by transporting lipids such as cholesterol.
  • Tissue specificity
    Highly expressed in lung, followed by brain, pancreas, skeletal muscle and heart. Weakly expressed in placenta, kidney and liver. Also expressed in medullary thyroid carcinoma cells (MTC) and in C-cell carcinoma.
  • Involvement in disease
    Defects in ABCA3 are the cause of pulmonary surfactant metabolism dysfunction type 3 (SMDP3) [MIM:610921]; also called pulmonary alveolar proteinosis due to ABCA3 deficiency. A rare lung disorder due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress.
  • Sequence similarities
    Belongs to the ABC transporter superfamily. ABCA family.
    Contains 2 ABC transporter domains.
  • Domain
    Multifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABC 3 antibody
    • ABC C antibody
    • ABC C transporter antibody
    • ABC transporter 3 antibody
    • ABC-C transporter antibody
    • ABC3 antibody
    • ABCA 3 antibody
    • Abca3 antibody
    • ABCA3 protein antibody
    • ABCA3_HUMAN antibody
    • ABCC antibody
    • ATP binding cassette 3 antibody
    • ATP binding cassette sub family A (ABC1) member 3 antibody
    • ATP binding cassette sub family A member 3 antibody
    • ATP binding cassette transporter 3 antibody
    • ATP-binding cassette 3 antibody
    • ATP-binding cassette sub-family A member 3 antibody
    • ATP-binding cassette transporter 3 antibody
    • CED7. C. elegans, homolog of antibody
    • EST111653 antibody
    • LBM 180 antibody
    • LBM180 antibody
    • MGC72201 antibody
    • P180 Lamellar Body Protein antibody
    • SMDP3 antibody
    see all

Images

  • ICC/IF image of ab99856 stained T47D cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab99856 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-ATP-binding cassette sub-family A member 3 antibody (ab99856) at 1/1000 dilution

    Lane 1 : SW480 cell lysate
    Lane 2 : Hela cell lysate
    Lane 3 : Human fetal kidney lysate
    Lane 4 : Human fetal lung lysate


    Predicted band size : 191 kDa

References

ab99856 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Sample
Rat Tissue sections (Rat Lung)
Specification
Rat Lung
Permeabilization
Yes - 0.1% Tween, 0.2% Triton
Fixative
Formaldehyde
Username

Stephanie Vadasz

Verified customer

Submitted Dec 04 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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