Anti-ATP-binding cassette sub-family A member 3 antibody (ab99856)


  • Product nameAnti-ATP-binding cassette sub-family A member 3 antibody
    See all ATP-binding cassette sub-family A member 3 primary antibodies
  • Description
    Rabbit polyclonal to ATP-binding cassette sub-family A member 3
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human ATP-binding cassette sub-family A member 3 aa 38-209.
    Database link: BC062779.1

  • Positive control
    • WB: Human fetal kidney and fetal lung tissue lysates and Hela cell lysate. ICC/IF: T47D


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab99856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/1000 - 1/2000. Predicted molecular weight: 191 kDa.


  • FunctionPlays an important role in the formation of pulmonary surfactant, probably by transporting lipids such as cholesterol.
  • Tissue specificityHighly expressed in lung, followed by brain, pancreas, skeletal muscle and heart. Weakly expressed in placenta, kidney and liver. Also expressed in medullary thyroid carcinoma cells (MTC) and in C-cell carcinoma.
  • Involvement in diseaseDefects in ABCA3 are the cause of pulmonary surfactant metabolism dysfunction type 3 (SMDP3) [MIM:610921]; also called pulmonary alveolar proteinosis due to ABCA3 deficiency. A rare lung disorder due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress.
  • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCA family.
    Contains 2 ABC transporter domains.
  • DomainMultifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABC 3 antibody
    • ABC C antibody
    • ABC C transporter antibody
    • ABC transporter 3 antibody
    • ABC-C transporter antibody
    • ABC3 antibody
    • ABCA 3 antibody
    • Abca3 antibody
    • ABCA3 protein antibody
    • ABCA3_HUMAN antibody
    • ABCC antibody
    • ATP binding cassette 3 antibody
    • ATP binding cassette sub family A (ABC1) member 3 antibody
    • ATP binding cassette sub family A member 3 antibody
    • ATP binding cassette transporter 3 antibody
    • ATP-binding cassette 3 antibody
    • ATP-binding cassette sub-family A member 3 antibody
    • ATP-binding cassette transporter 3 antibody
    • CED7. C. elegans, homolog of antibody
    • EST111653 antibody
    • LBM 180 antibody
    • LBM180 antibody
    • MGC72201 antibody
    • P180 Lamellar Body Protein antibody
    • SMDP3 antibody
    see all

Anti-ATP-binding cassette sub-family A member 3 antibody images

  • ICC/IF image of ab99856 stained T47D cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab99856 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-ATP-binding cassette sub-family A member 3 antibody (ab99856) at 1/1000 dilution

    Lane 1 : SW480 cell lysate
    Lane 2 : Hela cell lysate
    Lane 3 : Human fetal kidney lysate
    Lane 4 : Human fetal lung lysate

    Predicted band size : 191 kDa

References for Anti-ATP-binding cassette sub-family A member 3 antibody (ab99856)

ab99856 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Sample Rat Tissue sections (Rat Lung)
Specification Rat Lung
Permeabilization Yes - 0.1% Tween, 0.2% Triton
Fixative Formaldehyde

Stephanie Vadasz

Verified customer

Submitted Dec 04 2014