Anti-ATP citrate lyase antibody [3G8] (ab118150)

Overview

  • Product nameAnti-ATP citrate lyase antibody [3G8]
    See all ATP citrate lyase primary antibodies
  • Description
    Mouse monoclonal [3G8] to ATP citrate lyase
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human ATP citrate lyase produced in HEK293T cells (NP_001087).

  • Positive control
    • HEK293T cell lysate transfected with pCMV6-ENTRY ATP citrate lyase transfected; Human breast adenocarcinoma, colon, colon adenocarcinoma, kidney, liver, liver carcinoma, lung, ovary adenocarcinoma, pancreas, pancreas carcinoma, thyroid carcinoma, endometrium, endometrium adenocarcinoma, prostate, prostate carcinoma, and bladder tissies; COS7 cells transiently transfected by pCMV6-ENTRY ATP citrate lyase.
  • General notesDilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

Properties

Applications

Our Abpromise guarantee covers the use of ab118150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 120 kDa.
IHC-P 1/50.
ICC/IF 1/100.

Target

  • FunctionATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.
  • Sequence similaritiesIn the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.
    In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.
    Contains 1 ATP-grasp domain.
  • Post-translational
    modifications
    ISGylated.
    Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2.
    Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACL antibody
    • Acly antibody
    • ACLY_HUMAN antibody
    • ATP citrate (pro-S) lyase antibody
    • ATP citrate lyase antibody
    • ATP citrate synthase antibody
    • ATP-citrate (pro-S-)-lyase antibody
    • ATP-citrate synthase antibody
    • ATPcitrate synthase antibody
    • ATPCL antibody
    • Citrate cleavage enzyme antibody
    • CLATP antibody
    • OTTHUMP00000164773 antibody
    see all

Anti-ATP citrate lyase antibody [3G8] images

  • All lanes : Anti-ATP citrate lyase antibody [3G8] (ab118150) at 1/2000 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY ATP citrate lyase cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 120 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200508)
  • ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human breast adenocarcinoma tissue by Immunohistochemistry.
  • ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human colon tissue by Immunohistochemistry.
  • ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human colon adenocarcinoma tissue by Immunohistochemistry.
  • ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human kidney tissue by Immunohistochemistry.
  • ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human liver tissue by Immunohistochemistry.
  • ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human liver carcinoma tissue by Immunohistochemistry.
  • ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human lung tissue by Immunohistochemistry.
  • ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human ovary adenocarcinoma tissue by Immunohistochemistry.
  • ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human pancreas tissue by Immunohistochemistry.
  • ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human pancreas carcinoma tissue by Immunohistochemistry..
  • ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human thyroid tissue by Immunohistochemistry.
  • ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human endometrium tissue by Immunohistochemistry.
  • ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.
  • ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human prostate tissue by Immunohistochemistry.
  • ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human prostate carcinoma tissue by Immunohistochemistry..
  • ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human bladder tissue by Immunohistochemistry.
  • Immunofluorescent staining of COS7 cells transiently transfected by ATP citrate lyase using ab118150 at a dilution of 1/100.

References for Anti-ATP citrate lyase antibody [3G8] (ab118150)

ab118150 has not yet been referenced specifically in any publications.

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