Overview

  • Product name
    Anti-ATP citrate lyase antibody
    See all ATP citrate lyase primary antibodies
  • Description
    Rabbit polyclonal to ATP citrate lyase
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Pig, Chimpanzee, Zebrafish, Macaque monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human ATP citrate lyase.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Human Brain; Human testis; Human Skin; Human Liver; as well as the following whole cell lysates: PC3; HepG2; HEK293.

Properties

Applications

Our Abpromise guarantee covers the use of ab117239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 121 kDa (predicted molecular weight: 121 kDa).

Target

  • Function
    ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.
  • Sequence similarities
    In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.
    In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.
    Contains 1 ATP-grasp domain.
  • Post-translational
    modifications
    ISGylated.
    Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2.
    Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACL antibody
    • Acly antibody
    • ACLY_HUMAN antibody
    • ATP citrate (pro-S) lyase antibody
    • ATP citrate lyase antibody
    • ATP citrate synthase antibody
    • ATP-citrate (pro-S-)-lyase antibody
    • ATP-citrate synthase antibody
    • ATPcitrate synthase antibody
    • ATPCL antibody
    • Citrate cleavage enzyme antibody
    • CLATP antibody
    • OTTHUMP00000164773 antibody
    see all

Images

  • All lanes : Anti-ATP citrate lyase antibody (ab117239) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human testis tissue lysate - total protein (ab30257)
    Lane 3 : Human skin tissue lysate - total protein (ab30166)
    Lane 4 : Human liver tissue lysate - total protein (ab29889)
    Lane 5 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate
    Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 7 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 121 kDa
    Observed band size : 121 kDa
    Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

References

ab117239 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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