This antibody gave a positive signal in the following tissue lysates: Human Brain; Human testis; Human Skin; Human Liver; as well as the following whole cell lysates: PC3; HepG2; HEK293.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 121 kDa (predicted molecular weight: 121 kDa).
FunctionATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.
Sequence similaritiesIn the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family. In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family. Contains 1 ATP-grasp domain.
Post-translational modificationsISGylated. Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2. Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site.
Western blot - Anti-ATP citrate lyase antibody (ab117239)
All lanes : Anti-ATP citrate lyase antibody (ab117239) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466) Lane 2 : Human testis tissue lysate - total protein (ab30257) Lane 3 : Human skin tissue lysate - total protein (ab30166) Lane 4 : Human liver tissue lysate - total protein (ab29889) Lane 5 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 7 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 121 kDa Observed band size : 121 kDa Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 4 minutes
References for Anti-ATP citrate lyase antibody (ab117239)
has not yet been referenced specifically in any publications.
Publishing research using ab117239? Please let us know so that we can cite the reference in this datasheet.
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