Recombinant
RabMAb

Anti-ATP citrate lyase antibody [EP704Y] (ab40793)

Abreviews (2)Q&A (3)Specific References (13)
Customer reviews and FAQs

Overview

  • Product name
    Anti-ATP citrate lyase antibody [EP704Y]
    See all ATP citrate lyase primary antibodies
  • Description
    Rabbit monoclonal [EP704Y] to ATP citrate lyase
  • Specificity
    ab40793 recognises ATP citrate lyase (ACL).
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WB, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Common marmoset
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ATP citrate lyase aa 1050 to the C-terminus (C terminal). The exact sequence is proprietary.
    (Peptide available as ab207504)

  • Positive control
    • WB: HeLa cell lysate. ICC/IF: HeLa cells. Flow Cyt: HeLa cells. IP: Jurkat cell lysate.
  • General notes

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab40793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
IHC-P 1/100. PubMed: 19727777
WB 1/1000 - 1/5000. Detects a band of approximately 125 kDa (predicted molecular weight: 122 kDa).
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.

Target

  • Function
    ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.
  • Sequence similarities
    In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.
    In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.
    Contains 1 ATP-grasp domain.
  • Post-translational
    modifications
    ISGylated.
    Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2.
    Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • ACL antibody
    • Acly antibody
    • ACLY_HUMAN antibody
    • ATP citrate (pro-S) lyase antibody
    • ATP citrate lyase antibody
    • ATP citrate synthase antibody
    • ATP-citrate (pro-S-)-lyase antibody
    • ATP-citrate synthase antibody
    • ATPcitrate synthase antibody
    • ATPCL antibody
    • Citrate cleavage enzyme antibody
    • CLATP antibody
    • OTTHUMP00000164773 antibody
    see all

Anti-ATP citrate lyase antibody [EP704Y] images

  • Western blot - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
    Western blot - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
    Anti-ATP citrate lyase antibody [EP704Y] (ab40793) at 1/5000 dilution + HeLa cell lysate

    Predicted band size : 122 kDa
    Observed band size : 122 kDa
  • Immunocytochemistry/ Immunofluorescence - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
    Immunocytochemistry/ Immunofluorescence - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling ATP citrate lyase with ab40793 at 1/500. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Control: PBS only.
    Nuclear counter stain: DAPI.

  • Flow Cytometry - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
    Flow Cytometry - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)

    Overlay histogram showing HeLa cells stained with ab40793 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40793, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunoprecipitation - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
    Immunoprecipitation - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)

    ab40793 at 1/40 immunoprecipitating ATP citrate lyase in HeLa (human cervix adenocarcinoma) whole cell lysate.

    Lane 1 (input): HeLa (human cervix adenocarcinoma) whole cell lysate 10μg

    Lane 2 (+): ab40793 + HeLa (human cervix adenocarcinoma) whole cell lysate

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab40793 in HeLa (human cervix adenocarcinoma) whole cell lysate

    For western blotting, ab40793 at 1/1000 dilution and ab131366 VeriBlot for IP (HRP) was used as the secondary antibody at 1/10000.

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

References for Anti-ATP citrate lyase antibody [EP704Y] (ab40793)

This product has been referenced in:
  • Chu DT  et al. C57BL/6J mice as a polygenic developmental model of diet-induced obesity. Physiol Rep 5:N/A (2017). WB ; Mouse . Read more (PubMed: 28400497) »
  • Zhang C  et al. Cullin3-KLHL25 ubiquitin ligase targets ACLY for degradation to inhibit lipid synthesis and tumor progression. Genes Dev 30:1956-70 (2016). WB ; Human . Read more (PubMed: 27664236) »

See all 13 Publications for this product

Publishing research using ab40793? Please let us know so that we can cite the reference in this datasheet.

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Western blot abreview for Anti-ATP citrate lyase antibody [EP704Y]

 Good
Abreviews
Application
Western blot
Loading amount
80 µg
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Sample
Marmoset (common) Tissue lysate - whole (brain)
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
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Username

Abcam user community

Verified customer

Submitted Jun 05 2014

What's the concentration?

Vielen Dank für Ihren Anruf.

Leider ermitteln wir für diesen Antikörper die Konzentration nicht, so dass ich Ihnen leider in diesem Fall nicht weiterhelfen kann.

Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie...

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Western blot abreview for Anti-ATP citrate lyase antibody [EP704Y]

 Good
Abreviews
Application
Western blot
Sample
Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (Head)
Loading amount
40 µg
Specification
Head
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
abreview image
Username

Abcam user community

Verified customer

Submitted Oct 02 2012

How are you?

I am almost ready to submit the blots showing it worked. Whatexactlyshould I send you so I could get two new antibodies for free?


Please log into your Abcam account, and than open the datasheet for the antibody you would like to write a review about. On top of the datasheet, you will find five tags : Datasheet, specific references, Abreview, Scientific Support, Protocols. Read More

,


Thank you very much!

The first antibody is :

Anti-Histone H4 (acetyl K12) antibody (ab61238)
Which appears to also work on WB Drosophila.


The second is:

Anti-ATP citrate lyase an...

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Expiration date: 08. September 2012

ab40793 -DISCOUNT CODE: xxxx


ab61238- DISCOUNT CODE: xxxx

I am very pleased to hear you accepted our offer and tested ab40793 und ab61238 in drosophila. This code will give you: 1 f...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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