Anti-ATP citrate lyase (phospho S455) antibody [EP828Y] (ab46796)

Overview

  • Product name
    Anti-ATP citrate lyase (phospho S455) antibody [EP828Y]
    See all ATP citrate lyase primary antibodies
  • Description
    Rabbit monoclonal [EP828Y] to ATP citrate lyase (phospho S455)
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues of ATP citrate lyase (Human).

  • Positive control
    • Insulin treated 3T3 cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab46796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 125 kDa.
IP 1/20.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function
      ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.
    • Sequence similarities
      In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.
      In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.
      Contains 1 ATP-grasp domain.
    • Post-translational
      modifications
      ISGylated.
      Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2.
      Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • ACL antibody
      • Acly antibody
      • ACLY_HUMAN antibody
      • ATP citrate (pro-S) lyase antibody
      • ATP citrate lyase antibody
      • ATP citrate synthase antibody
      • ATP-citrate (pro-S-)-lyase antibody
      • ATP-citrate synthase antibody
      • ATPcitrate synthase antibody
      • ATPCL antibody
      • Citrate cleavage enzyme antibody
      • CLATP antibody
      • OTTHUMP00000164773 antibody
      see all

    Images

    • All lanes : Anti-ATP citrate lyase (phospho S455) antibody [EP828Y] (ab46796) at 1/5000 dilution

      Lane 1 : Untreated 3T3 cell lysates
      Lane 2 : Insulin treated 3T3 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Observed band size : 125 kDa (why is the actual band size different from the predicted?)

    References

    ab46796 has not yet been referenced specifically in any publications.

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