Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007)

Overview

  • Product nameAnti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y]
    See all ATP citrate lyase primary antibodies
  • Description
    Rabbit monoclonal [EP737Y] to ATP citrate lyase (phospho T447 + S451)
  • Specificityab53007 only detects ATP-citrate lyase phosphorylated on Threonine 447 and Serine 451.
  • Tested applicationsSuitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic phospho-peptide corresponding to residues surrounding Thr447 and Ser451 of human ATP-citrate (pro-S) lyase.

  • Positive control
    • 3T3 cytoplasmic cell lysate HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab53007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 122 kDa).
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
  • Application notesIs unsuitable for Flow Cyt or IHC-P.
  • Target

    • FunctionATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.
    • Sequence similaritiesIn the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.
      In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.
      Contains 1 ATP-grasp domain.
    • Post-translational
      modifications
      ISGylated.
      Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2.
      Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • ACL antibody
      • Acly antibody
      • ACLY_HUMAN antibody
      • ATP citrate (pro-S) lyase antibody
      • ATP citrate lyase antibody
      • ATP citrate synthase antibody
      • ATP-citrate (pro-S-)-lyase antibody
      • ATP-citrate synthase antibody
      • ATPcitrate synthase antibody
      • ATPCL antibody
      • Citrate cleavage enzyme antibody
      • CLATP antibody
      • OTTHUMP00000164773 antibody
      see all

    Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] images

    • All lanes : Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007) at 1/1000 dilution

      Lane 1 : (A) 3T3 cell lysate (untreated cells)
      Lane 2 : (B) 3T3 cell lysate (insulin treated cells)

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP labelled at 1/2000 dilution

      Predicted band size : 122 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    • ab53007 stained HeLa cells at 1/100-1/250 dilution.

    References for Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007)

    This product has been referenced in:
    • Grunert T  et al. A comparative proteome analysis links tyrosine kinase 2 (Tyk2) to the regulation of cellular glucose and lipid metabolism in response to poly(I:C). J Proteomics 74:2866-80 (2011). WB . Read more (PubMed: 21787891) »
    • Chu KY  et al. ATP-citrate lyase reduction mediates palmitate-induced apoptosis in pancreatic beta cells. J Biol Chem 285:32606-15 (2010). Read more (PubMed: 20693577) »

    See all 2 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab53007.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"