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ab109714 ATP synthase Enzyme Activity Microplate Assay Kit is used to determine the activity of ATP synthase (Complex V) in a human or rat sample. The ATP synthase enzyme is immunocaptured within the wells of the microplate and the enzyme activity is measured by monitoring the decrease in absorbance at 340 nm. Specifically, the conversion of ATP to ADP by ATP synthase is coupled to the oxidation reaction of NADH to NAD+ with a reduction in absorbance at 340 nm.
Store Buffer (Tube 1), Detergent, and the covered microplate at 4°C. Store the Lipid Mix and Reagent Mix at -80°C. For multiple experiments the Reagent Mix may be aliquoted at -80°C.
|ATP synthase precoated Microtiter plate||1 unit|
|Buffer||Tube 1||1 x 10ml|
|Detergent||1 x 1ml|
|Phospholipid||1 x 6ml|
|Reagent Mix||1 x 20ml|
Our Abpromise guarantee covers the use of ab109714 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|