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250 µg, 500 µg or 750 µg of monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture up to ~125 µg, ~250 µg or ~500 µg respectively of ATP synthase (Complex V) from heart mitochondria.
ab109715 allows isolation of the ATP synthase complex from small amounts of tissue. This facilitates subsequent analysis of assembly state and activity. Thus the enzyme retains olygomycin sensitive ATP hydrolysis activities after isolation. Finally, the extent of post translational modifications including oxidative damage can be readily analyzed by proteomic approaches or antibody detection of these modifications. Potential uses for ab109715 include but are not limited to examining alterations of ATP synthase subunits in inherited mitochondrial diseases, Alzheimer's disease, angiogenesis, hypertension and aging.
Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).
|Components||250 µg||500 µg||750 µg|
|ATP synthase monoclonal antibody coupled agarose beads||1 x 250µg||1 x 500µg||1 x 750µg|
Our Abpromise guarantee covers the use of ab109715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent dilution.|
Using the immunoprecipitation protocol provided, ATP synthase was isolated from various samples by antibody ab109867 crosslinked to protein G-agarose beads (ab109715). Protein bands identities were confirmed by mass spectrometry.