Overview

  • Product nameAnti-ATP10D antibody
    See all ATP10D primary antibodies
  • Description
    Rabbit polyclonal to ATP10D
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within the C terminal amino acids 1151-1200 (NLLFTSAPPV IYGVLEKDVS AETLMQLPEL YRSGQKSEAY LPHTFWITLL) of Human ATP10D (NP_065186).

  • Positive control
    • Fetal liver lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab81174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65, >168 kDa (predicted molecular weight: 160 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/1562500.

Target

  • RelevanceThe exact function of ATP10D remains unknown.
  • Cellular localizationCell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane. Note: Exit from the endoplasmic reticulum requires the presence of TMEM30A, but not that of TMEM30B.
  • Database links
  • Alternative names
    • ATP10D antibody
    • ATPase class V type 10D antibody
    • ATPVD antibody
    • KIAA1487 antibody
    • Probable phospholipid transporting ATPase VD antibody
    • Putative type IV aminophospholipid transporting ATPase antibody
    • Type IV aminophospholipid transporting ATPase antibody
    see all

Anti-ATP10D antibody images

  • Anti-ATP10D antibody (ab81174) at 1 µg/ml + fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 160 kDa
    Observed band size : >168 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

References for Anti-ATP10D antibody (ab81174)

ab81174 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"