The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Involvement in disease
Defects in ATP13A2 are the cause of Kufor-Rakeb syndrome (KRS) [MIM:606693]; also known as Parkinson disease type 9 (PARK9). KRS is a rare hereditary disease with juvenile onset. In addition to typical signs of Parkinson disease, affected individuals show symptoms of more widespread neurodegeneration, including dementia.
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.
Ab43075 staining ATP13A2 by immunohistochemistry using frozen tissue.
The cryostat section of the rat spinal cord was incubated in ab43075 to the human ATPase protein at the dilution of 1:1000 overnight followed with a biotinylated secondary antibody and ABC kit. The section was developed in diaminobenzidine solution and enhanced in the presence of nickel sulphate. Cell bodies and nerve fibers in the trigeminal nerve are stained.