Overview

  • Product nameAnti-ATP1B3 antibody
    See all ATP1B3 primary antibodies
  • Description
    Mouse polyclonal to ATP1B3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: all Mammals
  • Immunogen

    Full length human ATP1B3 protein

  • Positive control
    • ATP1B3 transfected 293T lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).

Target

  • FunctionThis is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-3 subunit is not known.
  • Sequence similaritiesBelongs to the X(+)/potassium ATPases subunit beta family.
  • Cellular localizationMembrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • AT1B3_HUMAN antibody
    • atp1b3 antibody
    • ATPase Na+/K+ transporting beta 3 polypeptide antibody
    • ATPB 3 antibody
    • ATPB-3 antibody
    • ATPB3 antibody
    • CD 298 antibody
    • CD298 antibody
    • CD298 antigen antibody
    • FLJ29027 antibody
    • Na K ATPase beta 3 polypeptide antibody
    • Na+/K+ ATPase beta 3 subunit antibody
    • NKAB3S antibody
    • Sodium potassium ATPase subunit beta 3 (non catalytic) antibody
    • Sodium pump subunit beta 3 antibody
    • Sodium/potassium dependent ATPase beta 3 subunit antibody
    • Sodium/potassium dependent ATPase subunit beta 3 antibody
    • Sodium/potassium transporting ATPase beta 3 chain antibody
    • Sodium/potassium transporting ATPase subunit beta 3 antibody
    • Sodium/potassium-dependent ATPase subunit beta-3 antibody
    • Sodium/potassium-transporting ATPase subunit beta-3 antibody
    see all

Anti-ATP1B3 antibody images

  • All lanes : Anti-ATP1B3 antibody (ab67409) at 1/500 dilution

    Lane 1 : ATP1B3 293T transfected lysate
    Lane 2 : Non transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 31 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 36 kDa. We are unsure as to the identity of these extra bands.

References for Anti-ATP1B3 antibody (ab67409)

ab67409 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TritonX-100
Blocking step 1%BSA, 5% goat serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
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Submitted Aug 26 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"