![Immunocytochemistry/ Immunofluorescence - Anti-ATP5A antibody [15H4C4] (FITC) (ab119688) image](#ATP5A-Primary-antibodies-ab119688-3.jpg)
![Flow Cytometry - Anti-ATP5A antibody [15H5C4] (FITC) (ab119688) image](#ATP5A-Primary-antibodies-ab119688-2.jpg)
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Product name
Anti-ATP5A antibody [15H4C4] (FITC)
See all ATP5A products (5) ...
Description
Mouse monoclonal [15H4C4] to ATP5A (FITC)
Conjugation
FITC
Conjugation notes
Fluorochrome/Protein Ratio: at least 3 moles FITC per mole of IgG
Tested applications
WB, Flow Cyt, ICCmore details
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human, Caenorhabditis elegans, Fruit fly (Drosophila melanogaster), Monkey, Other
Immunogen
Bovine Complex V
General notes
Absorption Wavelength: 495 nm
Emission Wavelength: 528 nm
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Purification notes
Purity is near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation.
Clonality
Monoclonal
Clone number
15H4C4
Isotype
IgG2b
Light chain type
kappa
Applications
Show applications key
Our Abpromise guarantee covers the use of ab119688 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent concentration. Predicted molecular weight: 60 kDa.
Flow Cyt: Use a concentration of 1 µg/ml
ICC: Use a concentration of 1 µg/ml
Target
Function
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites.
Tissue specificity
Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord.
Sequence similarities
Belongs to the ATPase alpha/beta chains family.
Post-translational
modifications
The N-terminus is blocked.
Cellular localization
Mitochondrion inner membrane. Peripheral membrane protein.
Target information above from: UniProt accessionP25705
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mitochondrial antibody
- ATP synthase alpha chain, mitochondrial antibody
- ATP synthase subunit alpha antibody
see all
Database links
- Entrez Gene: 282578 Cow
- Entrez Gene: 37617 Fruit fly (Drosophila melanogaster)
- Entrez Gene: 498 Human
- Entrez Gene: 11946 Mouse
- Entrez Gene: 65262 Rat
- Omim: 164360 Human
- SwissProt: P19483 Cow
- SwissProt: P35381 Fruit fly (Drosophila melanogaster)
see all
Anti-ATP5A antibody [15H4C4] (FITC) images:
Immunocytochemistry/ Immunofluorescence - Anti-ATP5A antibody [15H4C4] (FITC) (ab119688)
![Immunocytochemistry/ Immunofluorescence - Anti-ATP5A antibody [15H4C4] (FITC) (ab119688)](/ps/datasheet/images/119/ab119688/ATP5A-Primary-antibodies-ab119688-3.jpg)
Mitochondrial localization of ATP5A using antibody ab119688. Cultured HeLa cells were fixed, permeabilized and then labeled with 15H4C4-FITC (1 µg/ml). Since the antibody is labeled with FITC no secondary antibody is necessary.
Flow Cytometry - Anti-ATP5A antibody [15H5C4] (FITC) (ab119688)
![Flow Cytometry - Anti-ATP5A antibody [15H5C4] (FITC) (ab119688)](/ps/datasheet/images/119/ab119688/ATP5A-Primary-antibodies-ab119688-2.jpg)
Overlay histogram showing HeLa cells stained with ab119688 (blue line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody ab119688 (15H4C4 FITC) at 1µg/1xE6/mL cells for 30 min at 22ºC. Negative control (red line) is unstained d cells. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
References for Anti-ATP5A antibody [15H4C4] (FITC) (ab119688)
ab119688 has not yet been referenced specifically in any publications.
Publishing research using ab119688? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"