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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
Anti-ATP5A antibody [15H4C4] (FITC) - Mitochondrial Marker (ab119688)
MS Catalog No. MS1028
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab119688? Please let us know so that we can cite the reference in this datasheet
ab119688 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Immunocytochemistry/ Immunofluorescence - Anti-ATP5A antibody [15H4C4] (FITC) (ab119688)](/ps/datasheet/images/119/ab119688/ATP5A-Primary-antibodies-ab119688-3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ATP5A antibody [15H4C4] (FITC) (ab119688)
Mitochondrial localization of ATP5A using antibody ab119688. Cultured HeLa cells were fixed, permeabilized and then labeled with 15H4C4-FITC (1 µg/ml). Since the antibody is labeled with FITC no secondary antibody is necessary.
![Flow Cytometry - Anti-ATP5A antibody [15H5C4] (FITC) (ab119688)](/ps/datasheet/images/119/ab119688/ATP5A-Primary-antibodies-ab119688-2.jpg)
Flow Cytometry - Anti-ATP5A antibody [15H5C4] (FITC) (ab119688)
Overlay histogram showing HeLa cells stained with ab119688 (blue line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody ab119688 (15H4C4 FITC) at 1µg/1xE6/mL cells for 30 min at 22ºC. Negative control (red line) is unstained d cells. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
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