Overview

  • Product name
  • Description
    Rabbit polyclonal to ATP5A
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide, conjugated to KLH, from internal sequence amino acids of Human ATP5A (NP_004037.1).

  • Positive control
    • Human breast and heart tissues; 293 and RAW264.7 cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab118482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 60 kDa.
IHC-P Use a concentration of 5 µg/ml.
ELISA 1/40000.

Target

  • Function
    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites.
  • Tissue specificity
    Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord.
  • Sequence similarities
    Belongs to the ATPase alpha/beta chains family.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localization
    Mitochondrion inner membrane. Peripheral membrane protein.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP synthase alpha chain, mitochondrial antibody
    • ATP synthase subunit alpha antibody
    • ATP synthase subunit alpha mitochondrial antibody
    • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle antibody
    • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, 1 antibody
    • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle antibody
    • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2 antibody
    • ATP sythase (F1 ATPase) alpha subunit antibody
    • ATP5A antibody
    • Atp5a1 antibody
    • ATP5AL2 antibody
    • ATPA_HUMAN antibody
    • ATPM antibody
    • Epididymis secretory sperm binding protein Li 123m antibody
    • hATP1 antibody
    • HEL-S-123m antibody
    • MC5DN4 antibody
    • mitochondrial antibody
    • Mitochondrial ATP synthetase antibody
    • Mitochondrial ATP synthetase oligomycin resistant antibody
    • Modifier of Min 2 mouse homolog antibody
    • Modifier of Min 2, mouse, homolog of antibody
    • MOM2 antibody
    • OMR antibody
    • ORM antibody
    • OTTHUMP00000163475 antibody
    see all

Images

  • ab118482 at 5µg/ml staining ATP5A in Formalin-fixed, Paraffin-embedded Human breast tissue by immunohistochemistry.
  • ab118482 at 5µg/ml staining ATP5A in Formalin-fixed, Paraffin-embedded Human heart tissue by immunohistochemistry.
  • All lanes : Anti-ATP5A antibody (ab118482) at 1/500 dilution

    Lane 1 : RAW264.7 cell extract
    Lane 2 : 293 cell extract
    Lane 3 : RAW264.7 cell extract with sythesized immunizing peptide


    Predicted band size : 60 kDa

References

ab118482 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us.

I have been in touch with the lab regarding the sequence homology of the immunogens for the anti-ATP5A products and E.coli.

ab136082: The immunogen is aa200-250 of human ATP synthase subunit alpha with a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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