• Product name
    Anti-ATP5D antibody [4D11]
    See all ATP5D primary antibodies
  • Description
    Mouse monoclonal [4D11] to ATP5D
  • Host species
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow
    Does not react with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Cow ATP5D. Mitochondrial fraction from Bovine Heart

  • Positive control
    • Rat and mouse heart and liver homogenates, rat H4IIE lysate and mouse MEF cell lysate.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.



Our Abpromise guarantee covers the use of ab174438 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.


  • Function
    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP turnover in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
  • Sequence similarities
    Belongs to the ATPase epsilon chain family.
  • Cellular localization
    Mitochondrion. Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP synthase subunit delta, mitochondrial antibody
    • ATP synthase subunit delta, mitochondrial antibody
    • ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit antibody
    • ATP5D antibody
    • ATPD_HUMAN antibody
    • F ATPase delta subunit antibody
    • F-ATPase delta subunit antibody
    • Mitochondrial ATP synthase complex delta subunit precusor antibody
    • Mitochondrial ATP synthase delta subunit antibody
    see all


  • All lanes : Anti-ATP5D antibody [4D11] (ab174438) at 1 µg/ml (5% milk PBST)

    Lane 1 : Human Heart Homogenate at 10 µg
    Lane 2 : Human Liver Homogenate at 10 µg
    Lane 3 : Rat Liver Homogenate at 10 µg
    Lane 4 : Mouse Liver Homogenate at 10 µg
    Lane 5 : Bovine Heart Homogenate at 10 µg
    Lane 6 : Human HepG2 cell lysate at 20 µg
    Lane 7 : Rat H4IIE cell lysate at 20 µg
    Lane 8 : Mouse MEF cell lysate at 20 µg

    All lanes : Goat anti Mouse IR700 in 5% milk/PBST at 1/5000 dilution

    Predicted band size: 17 kDa


ab174438 has not yet been referenced specifically in any publications.

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