Overview

  • Product nameAnti-ATP5D antibody
    See all ATP5D primary antibodies
  • Description
    Rabbit polyclonal to ATP5D
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide between amino acids 1-30 from the N terminal region of Human ATP5D (NP_001678.1, NP_001001975.1).

  • Positive control
    • MDA-MB435 cell lysate; Human hepatocarcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab107077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 17 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP turnover in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
  • Sequence similaritiesBelongs to the ATPase epsilon chain family.
  • Cellular localizationMitochondrion. Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP synthase subunit delta, mitochondrial antibody
    • ATP synthase subunit delta, mitochondrial antibody
    • ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit antibody
    • ATP5D antibody
    • ATPD_HUMAN antibody
    • F ATPase delta subunit antibody
    • F-ATPase delta subunit antibody
    • Mitochondrial ATP synthase complex delta subunit precusor antibody
    • Mitochondrial ATP synthase delta subunit antibody
    see all

Anti-ATP5D antibody images

  • Anti-ATP5D antibody (ab107077) at 1/100 dilution + MDA-MB435 cell lysate at 35 µg

    Predicted band size : 17 kDa
  • ab107077, at a 1/50 dilution, staining ATP5D in formalin fixed, paraffin embedded Human hepatocacinoma by Immunohistochemistry, followed by peroxidase conjugation of the secondary antibody and DAB staining.

References for Anti-ATP5D antibody (ab107077)

ab107077 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"