Anti-ATP5F1 antibody (ab154564)
Key features and details
- Rabbit polyclonal to ATP5F1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ATP5F1 antibody
See all ATP5F1 primary antibodies -
Description
Rabbit polyclonal to ATP5F1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 66-256 of Human ATP5F1 (Uniprot ID: P24539).
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Positive control
- MOLT4, Mouse brain whole cell lysate; HeLa cells; Human colon tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 29 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 29 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. -
Sequence similarities
Belongs to the eukaryotic ATPase B chain family. -
Cellular localization
Mitochondrion. Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 515 Human
- Entrez Gene: 11950 Mouse
- Omim: 603270 Human
- SwissProt: P24539 Human
- SwissProt: Q9CQQ7 Mouse
- Unigene: 514870 Human
- Unigene: 251152 Mouse
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Alternative names
- AT5F1_HUMAN antibody
- ATP synthase B chain mitochondrial antibody
- ATP synthase subunit b antibody
see all
Images
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Anti-ATP5F1 antibody (ab154564) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg
Predicted band size: 29 kDa
12% SDS PAGE -
Anti-ATP5F1 antibody (ab154564) at 1/500 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 29 kDa
12% SDS PAGE -
Immunofluorescent analysis of methanol-fixed HeLa cells labeling ATP5F1 with ab154564 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
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Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling ATP5F1 with ab154564 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154564 has not yet been referenced specifically in any publications.