Overview

  • Product name
  • Description
    Rabbit polyclonal to ATP5F1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 66-256 of Human ATP5F1 (Uniprot ID: P24539).

  • Positive control
    • MOLT4, Mouse brain whole cell lysate; HeLa cells; Human colon tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab154564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 29 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
  • Sequence similarities
    Belongs to the eukaryotic ATPase B chain family.
  • Cellular localization
    Mitochondrion. Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AT5F1_HUMAN antibody
    • ATP synthase B chain mitochondrial antibody
    • ATP synthase subunit b antibody
    • ATP synthase subunit b mitochondrial antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b, antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b, isoform 1 antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1 antibody
    • ATP5F1 antibody
    • ATPase subunit b antibody
    • Cell proliferation inducing protein 47 antibody
    • H+ ATP synthase subunit b antibody
    • MGC24431 antibody
    • mitochondrial antibody
    • PIG47 antibody
    see all

Images

  • Anti-ATP5F1 antibody (ab154564) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size: 29 kDa



    12% SDS PAGE
  • Anti-ATP5F1 antibody (ab154564) at 1/500 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 29 kDa



    12% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling ATP5F1 with ab154564 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling ATP5F1 with ab154564 at 1/500 dilution.

References

ab154564 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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