Overview

  • Product name
    Anti-ATP5F1 antibody
    See all ATP5F1 primary antibodies
  • Description
    Rabbit polyclonal to ATP5F1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 180-229 (VTYRERLYRV YKEVKNRLDY HISVQNMMRR KEQEHMINWV EKHVVQSIST) of human ATP5F1 (NP_001679).

  • Positive control
    • 721_B cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab84625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
  • Sequence similarities
    Belongs to the eukaryotic ATPase B chain family.
  • Cellular localization
    Mitochondrion. Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • AT5F1_HUMAN antibody
    • ATP synthase B chain mitochondrial antibody
    • ATP synthase subunit b antibody
    • ATP synthase subunit b mitochondrial antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b, antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b, isoform 1 antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1 antibody
    • ATP5F1 antibody
    • ATPase subunit b antibody
    • Cell proliferation inducing protein 47 antibody
    • H+ ATP synthase subunit b antibody
    • MGC24431 antibody
    • mitochondrial antibody
    • PIG47 antibody
    see all

Images

  • Western blot - ATP5F1 antibody (ab84625)
    Western blot - ATP5F1 antibody (ab84625)
    Anti-ATP5F1 antibody (ab84625) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 29 kDa
    Observed band size: 26 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 64 kDa. We are unsure as to the identity of these extra bands.

References

ab84625 has not yet been referenced specifically in any publications.

Publishing research using ab84625? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Western blot abreview for Anti-ATP5F1 antibody

 Good
Abreviews
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (7% GEL)
Sample
Human Cell lysate - other (H23)
Specification
H23
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Oct 31 2013

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