- Datasheet
- Specific References
- Protocols
Overview
- Product nameAnti-ATP5F1 antibody
See all ATP5F1 primary antibodies - DescriptionRabbit polyclonal to ATP5F1
- Tested applicationsSuitable for: WBmore details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
- Immunogen
Synthetic peptide corresponding to a region within internal sequence amino acids 180-229 (VTYRERLYRV YKEVKNRLDY HISVQNMMRR KEQEHMINWV EKHVVQSIST) of human ATP5F1 (NP_001679).
- Positive control
- 721_B cell lysate
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesPurified by peptide affinity chromatography method.
- ClonalityPolyclonal
- IsotypeIgG
- Research areas
Associated products
- Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab84625 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
Target
- FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
- Sequence similaritiesBelongs to the eukaryotic ATPase B chain family.
- Cellular localizationMitochondrion. Mitochondrion inner membrane.
- Information by UniProt
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Database links
- Entrez Gene: 515 Human
- Entrez Gene: 11950 Mouse
- Entrez Gene: 171375 Rat
- Omim: 603270 Human
- SwissProt: P24539 Human
- SwissProt: Q9CQQ7 Mouse
- SwissProt: P19511 Rat
- Unigene: 514870 Human
see all -
Alternative names
- AT5F1_HUMAN antibody
- ATP synthase B chain mitochondrial antibody
- ATP synthase subunit b antibody
see all
Anti-ATP5F1 antibody images
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Western blot - ATP5F1 antibody (ab84625)Anti-ATP5F1 antibody (ab84625) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 29 kDa
Observed band size : 26 kDa (why is the actual band size different from the predicted?)
Additional bands at : 64 kDa. We are unsure as to the identity of these extra bands.
References for Anti-ATP5F1 antibody (ab84625)
ab84625 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"