Anti-ATP5H antibody [7F9BG1] (ab110275)
Key features and details
- Mouse monoclonal [7F9BG1] to ATP5H
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Cow, Human, African green monkey
- Isotype: IgG2b
Overview
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Product name
Anti-ATP5H antibody [7F9BG1]
See all ATP5H primary antibodies -
Description
Mouse monoclonal [7F9BG1] to ATP5H -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human, African green monkey -
Immunogen
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Isolated mitochondria from Human heart, Bovine heart, Rat heart, Mouse heart, and HepG2, Cultured Human embryonic lung-derived fibroblasts (strain MRC5), HeLa cells
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General notes
For mouse and rat samples, this antibody will only recognize ATP5H in purified mitochondrial samples. Mouse and rat cell lysates and tissue homogenates are not recommneded with this antibody.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Near homogeneity as judged by SDS-PAGE. ab110275 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
7F9BG1 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110275 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 18 kDa.
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ICC/IF |
Use a concentration of 1 - 5 µg/ml.
(heat-induced antigen-retrieval improvessignal) |
|
Flow Cyt | (1) |
Use a concentration of 1 µg/ml.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 18 kDa. |
ICC/IF
Use a concentration of 1 - 5 µg/ml. (heat-induced antigen-retrieval improvessignal) |
Flow Cyt
Use a concentration of 1 µg/ml. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Function
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. -
Sequence similarities
Belongs to the ATPase d subunit family. -
Cellular localization
Mitochondrion. Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 282710 Cow
- Entrez Gene: 10476 Human
- Entrez Gene: 71679 Mouse
- Entrez Gene: 641434 Rat
- SwissProt: P13620 Cow
- SwissProt: O75947 Human
- SwissProt: Q9DCX2 Mouse
- SwissProt: P31399 Rat
see all -
Alternative names
- ATP synthase D chain mitochondrial antibody
- ATP synthase H+ transporting mitochondrial F1F0 subunit antibody
- ATP synthase H+ transporting mitochondrial F1F0 subunit d antibody
see all
Images
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All lanes : Anti-ATP5H antibody [7F9BG1] (ab110275) at 1 µg/ml
Lane 1 : HDFn (human) cell lysates at 20 µg
Lane 2 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysates at 20 µg
Lane 3 : H4IIE (rat) cell lysates at 20 µg
Lane 4 : MEF (mouse) cell lysates at 20 µg
Lane 5 : bovine heart mitochondria lysates at 5 µg
Predicted band size: 18 kDa -
All lanes : Anti-ATP5H antibody [7F9BG1] (ab110275) at 1/1 dilution
Lane 1 : Human heart mitochondria at 10 µg
Lane 2 : Bovine heart mitochondria at 4 µg
Lane 3 : Rat heart mitochondria at 10 µg
Lane 4 : Mouse heart mitochondria at 10 µg
Lane 5 : HepG2 mitochondria at 20 µg
Predicted band size: 18 kDa -
Mitochondrial localization of ATP5H. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with ab110275 (5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG(H+L) secondary antibody (2 µg/ml).
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HeLa cells were stained with 1 µg/mL ab110275 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab110275 has been referenced in 16 publications.
- Lewis CJ et al. Codon optimization is an essential parameter for the efficient allotopic expression of mtDNA genes. Redox Biol 30:101429 (2020). WB ; Human . PubMed: 31981894
- Ng R et al. Contractile work directly modulates mitochondrial protein levels in human engineered heart tissues. Am J Physiol Heart Circ Physiol 318:H1516-H1524 (2020). PubMed: 32383992
- Chaphalkar RM et al. Endothelin-1 Mediated Decrease in Mitochondrial Gene Expression and Bioenergetics Contribute to Neurodegeneration of Retinal Ganglion Cells. Sci Rep 10:3571 (2020). PubMed: 32107448
- Vilella R et al. Effects of Standardized Green Tea Extract and Its Main Component, EGCG, on Mitochondrial Function and Contractile Performance of Healthy Rat Cardiomyocytes. Nutrients 12:N/A (2020). PubMed: 32993022
- Pecina P et al. Role of the mitochondrial ATP synthase central stalk subunits ? and d in the activity and assembly of the mammalian enzyme. Biochim Biophys Acta 1859:374-381 (2018). PubMed: 29499186