Overview

  • Product nameAnti-ATP5H antibody [7F9BG1]
    See all ATP5H primary antibodies
  • Description
    Mouse monoclonal [7F9BG1] to ATP5H
  • Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, African Green Monkey
  • Immunogen

    Other Immunogen Type corresponding to Cow ATP5H.

  • Positive control
    • Isolated mitochondria from Human heart, Bovine heart, Rat heart, Mouse heart, and HepG2, Cultured Human embryonic lung-derived fibroblasts (strain MRC5), HeLa cells
  • General notes

    For mouse and rat samples, this antibody will only recognize ATP5H in purified mitochondrial samples. Mouse and rat cell lysates and tissue homogenates are not recommneded with this antibody.

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

Our Abpromise guarantee covers the use of ab110275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 18 kDa.
ICC/IF Use a concentration of 1 - 5 µg/ml. (heat-induced antigen-retrieval improves signal)
Flow Cyt Use a concentration of 1 µg/ml. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
  • Sequence similaritiesBelongs to the ATPase d subunit family.
  • Cellular localizationMitochondrion. Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP synthase D chain mitochondrial antibody
    • ATP synthase H+ transporting mitochondrial F1F0 subunit antibody
    • ATP synthase H+ transporting mitochondrial F1F0 subunit d antibody
    • ATP synthase subunit d antibody
    • ATP synthase subunit d, mitochondrial antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d antibody
    • ATP5H antibody
    • ATP5H_HUMAN antibody
    • ATP5JD antibody
    • ATPase subunit d antibody
    • ATPQ antibody
    • mitochondrial antibody
    • My032 protein antibody
    see all

Anti-ATP5H antibody [7F9BG1] images

  • All lanes : Anti-ATP5H antibody [7F9BG1] (ab110275) at 1 µg/ml

    Lane 1 : HDFn (human) cell lysates at 20 µg
    Lane 2 : COS7 (monkey) cell lysates at 20 µg
    Lane 3 : H4IIE (rat) cell lysates at 20 µg
    Lane 4 : MEF (mouse) cell lysates at 20 µg
    Lane 5 : bovine heart mitochondria lysates at 5 µg


    Predicted band size : 18 kDa
  • All lanes : Anti-ATP5H antibody [7F9BG1] (ab110275) at 1/1 dilution

    Lane 1 : Human heart mitochondria at 10 µg
    Lane 2 : Bovine heart mitochondria at 4 µg
    Lane 3 : Rat heart mitochondria at 10 µg
    Lane 4 : Mouse heart mitochondria at 10 µg
    Lane 5 : HepG2 mitochondria at 20 µg


    Predicted band size : 18 kDa
  • Mitochondrial localization of ATP5H. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with ab110275 (5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG(H+L) secondary antibody (2 µg/ml).
  • HeLa cells were stained with 1 µg/mL ab110275 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

References for Anti-ATP5H antibody [7F9BG1] (ab110275)

This product has been referenced in:
  • Boominathan A  et al. Stable nuclear expression of ATP8 and ATP6 genes rescues a mtDNA Complex V null mutant. Nucleic Acids Res N/A:N/A (2016). Read more (PubMed: 27596602) »
  • Mayr JA  et al. Mitochondrial ATP synthase deficiency due to a mutation in the ATP5E gene for the F1 epsilon subunit. Hum Mol Genet 19:3430-9 (2010). Read more (PubMed: 20566710) »

See all 8 Publications for this product

Product Wall



For this antibody we have used a protocol with PFA and then a permeabilisation step with TritonX:

1. Fix the cells by incubating them in 4% (v/v) paraformaldehyde in PBS for 20 minutes at room

temperature.

2. Rinse ...

Read More
Application Flow Cytometry
Fixation Beckman Coulter PerFix-nc
Permeabilization Yes - Beckman Coulter PerFix-nc
Sample Rhesus monkey Cell (MSC)
Specification MSC
Gating Strategy The initial gate is set for total bone marrow
Preparation Cell harvesting/tissue preparation method: Isolated and expanded MSCs
Sample buffer: MEM + 20% FBS
Username

Abcam user community

Verified customer

Submitted Oct 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK 29#T)
Loading amount 20 µg
Specification HEK 29#T
Gel Running Conditions Reduced Denaturing (10% Bis Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"