Overview

  • Product name
  • Description
    Rabbit polyclonal to ATP5I
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    antigen sequence:

    RRIAAEEKKKQDELKRIARELAEDDSILK

    , corresponding to amino acids 41-69 of Human ATP5I.

  • Positive control
    • Human stomach tissue; RT-4 and U-251 MG cell lysates; Human Liver and Tonsil lysates; Human cell line A-431
  • General notes


    ab122241 is mono-specific.

Properties

Applications

Our Abpromise guarantee covers the use of ab122241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Function
    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.
  • Sequence similarities
    Belongs to the ATPase e subunit family.
  • Cellular localization
    Mitochondrion. Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP 5I antibody
    • ATP 5K antibody
    • ATP synthase e chain mitochondrial antibody
    • ATP synthase H+ transporting mitochondrial F0 complex subunit E antibody
    • ATP synthase subunit e antibody
    • ATP synthase subunit e mitochondrial antibody
    • ATP5I antibody
    • ATP5I_HUMAN antibody
    • ATP5K antibody
    • ATPase subunit e antibody
    • F1F0 ATP synthase murine e subunit antibody
    • MGC12532 antibody
    • mitochondrial antibody
    see all

Images

  • ab122241, at 1/200 dilution, staining ATP5I in paraffin-embedded Human stomach tissue by Immunohistochemistry.
  • ab122241, at 4µg/ml, staining ATP5I in Human cell line A-431 by Immunofluorescence. Antibody staining is shown in green.
  • All lanes : Anti-ATP5I antibody (ab122241) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

References

ab122241 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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