MitoSciences (MS508)

Anti-ATP5J antibody [10C6AC9] (ab110279)

Overview

  • Product nameAnti-ATP5J antibody [10C6AC9]
    See all ATP5J primary antibodies
  • Description
    Mouse monoclonal [10C6AC9] to ATP5J
  • Tested applicationsWB, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Human ATP5J protein (UniProt ID: P18859).

  • Positive control
    • WB: Isolated mitochondria from Human heart lysate. ICc/IF: Cultured Human embryonic lung-derived fibroblasts (strain MRC5) Flow Cyt: HL-60 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab110279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 13 kDa.
Flow Cyt Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes.
  • Sequence similaritiesBelongs to the eukaryotic ATPase subunit F6 family.
  • Cellular localizationMitochondrion. Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 antibody
    • ATP synthase-coupling factor 6 antibody
    • ATP synthase-coupling factor 6, mitochondrial antibody
    • ATP5 antibody
    • ATP5A antibody
    • ATP5J antibody
    • ATP5J_HUMAN antibody
    • ATPase subunit F6 antibody
    • ATPM antibody
    • CF6 antibody
    • F6 antibody
    • mitochondrial antibody
    see all

Anti-ATP5J antibody [10C6AC9] images

  • Anti-ATP5J antibody [10C6AC9] (ab110279) at 1 µg/ml + Isolated mitochondria from Human heart lysate at 5 µg

    Predicted band size : 13 kDa
  • Mitochondrial localization of ATP synthase visualized by immunocytochemistry using ab110279.
    Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with ab110279 (1 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG(H+L) secondary antibody (2 µg/ml).
  • HL-60 cells were stained with 1 µg/mL ab110279 (blue) or an equal amount of an isotype control antibody (red) and analyzed by Flow Cytometry.
  • All lanes : Anti-ATP5J antibody [10C6AC9] (ab110279)

    Lane 1 : human heart homogenate at 20 µg
    Lane 2 : HepG2 cell lysate at 20 µg
    Lane 3 : human liver mitochondrial extraction at 10 µg
    Lane 4 : bovine heart mitochondrial extraction at 10 µg
    Lane 5 : human heart mitochondrial extraction at 10 µg
    Lane 6 : human liver homogenate at 20 µg


    Predicted band size : 13 kDa

References for Anti-ATP5J antibody [10C6AC9] (ab110279)

This product has been referenced in:
  • Mayr JA  et al. Mitochondrial ATP synthase deficiency due to a mutation in the ATP5E gene for the F1 epsilon subunit. Hum Mol Genet 19:3430-9 (2010). Read more (PubMed: 20566710) »

See 1 Publication for this product

Product Wall

Thank you for your email, I am sorry for the delay in getting back to you.

I have a couple of antibodies your customer may be interested in:

Anti-Frataxin antibody [18A5DB1] (ab110328)

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Anti-ATP5J antibody [10C6AC...

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Merci beaucoup pour votre patience. Le laboratoire avait retesté cet anticorps sur différents tissus. Veuillez trouver les résultats dans le pièce jointe. Effectivement nous sommes très confiantes que cet anticorps reconnaît le ATP5J, car il était déve...

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Je suis désolée d'apprendre que ce tube de l'ab110279 ne donne pas de bons résultats en Western blot.  Veuillez trouver ci-joint le questionnaire discuté au téléphone. Veuillez le nous retourner avec une image du résultat. Comme discuté aussi, je vais ...

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DISCOUNT CODE: XXXXXXXXX Expiration date: 15-01-2012 I am very pleased to hear you would like to accept our offer and test ab110279 in Mouse. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, pl...

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Thank you for contacting us. We have not tested this antibody with mouse lysates yet so we unfortunately are unable to share any tested data. The mouse and human protein have 80% homology so it is more likely that this antibody could detect the...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"