Overview

  • Product name
  • Description
    Rabbit polyclonal to ATP5J
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-108 of Human ATP5J (P18859).

  • Positive control
    • HeLa whole cell lysate and HeLa membrane lysate. Hela cells.
  • General notes
    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab154578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 12 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes.
  • Sequence similarities
    Belongs to the eukaryotic ATPase subunit F6 family.
  • Cellular localization
    Mitochondrion. Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 antibody
    • ATP synthase-coupling factor 6, mitochondrial antibody
    • ATP synthase-coupling factor 6, mitochondrial antibody
    • ATP5 antibody
    • ATP5A antibody
    • ATP5J antibody
    • ATP5J_HUMAN antibody
    • ATPase subunit F6 antibody
    • ATPM antibody
    • CF6 antibody
    • F6 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded J5 xenograft, labelling ATP5J using ab154578 at 1/500.
  • Immunofluorescence analysis of methanol-fixed HeLa cells, labelling ATP5J using: (1) ab154578 at 1/500, (2) Co-stained with Hoechst 33342.
  • All lanes : Anti-ATP5J antibody (ab154578) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HeLa membrane lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 12 kDa



    15% SDS PAGE

References

ab154578 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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