Overview

  • Product name
  • Description
    Rabbit polyclonal to ATP5J
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide mapping at the middle region of human mature ATP5J, different from the related mouse sequence by two amino acids.

  • Positive control
    • Mammary cancer tissue and rat liver tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab65841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notes
    IHC-P: Use at a concentration of 1 - 2 µg/ml. Boiling the sections is required.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 13 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes.
    • Sequence similarities
      Belongs to the eukaryotic ATPase subunit F6 family.
    • Cellular localization
      Mitochondrion. Mitochondrion inner membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 antibody
      • ATP synthase-coupling factor 6, mitochondrial antibody
      • ATP synthase-coupling factor 6, mitochondrial antibody
      • ATP5 antibody
      • ATP5A antibody
      • ATP5J antibody
      • ATP5J_HUMAN antibody
      • ATPase subunit F6 antibody
      • ATPM antibody
      • CF6 antibody
      • F6 antibody
      see all

    Images

    • Anti-ATP5J antibody (ab65841) at 1 µg/ml + Rat liver tissue lysate

      Predicted band size : 13 kDa
      Observed band size : 18 kDa (why is the actual band size different from the predicted?)
    • ab65841 at 2µg/ml staining ATP5J in Mammary cancer by Immunohistochemistry, Paraffin-embedded tissue (DAB chromogenic reaction).

    References

    This product has been referenced in:
    • Zeng Z  et al. Cyclic mechanical stretch promotes energy metabolism in osteoblast-like cells through an mTOR signaling-associated mechanism. Int J Mol Med 36:947-56 (2015). Read more (PubMed: 26251974) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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