Anti-ATP5J antibody (ab65841)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-ATP5J antibody
See all ATP5J primary antibodies -
DescriptionRabbit polyclonal to ATP5J
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
A synthetic peptide mapping at the middle region of human mature ATP5J, different from the related mouse sequence by two amino acids.
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Positive control
- Mammary cancer tissue and rat liver tissue lysate.
Properties
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FormLyophilised:0.2ml of distilled water will yield a concentration of 500µg/ml.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab65841 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| IHC-P |
Application notes
IHC-P: Use at a concentration of 1 - 2 µg/ml. Boiling the sections is required.
WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 13 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 13 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes.
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Sequence similaritiesBelongs to the eukaryotic ATPase subunit F6 family.
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Cellular localizationMitochondrion. Mitochondrion inner membrane.
- Information by UniProt
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Database links
- Entrez Gene: 522 Human
- Entrez Gene: 11957 Mouse
- Entrez Gene: 94271 Rat
- Omim: 603152 Human
- SwissProt: P18859 Human
- SwissProt: P97450 Mouse
- SwissProt: P21571 Rat
- Unigene: 246310 Human
see all -
Alternative names
- ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 antibody
- ATP synthase-coupling factor 6, mitochondrial antibody
- ATP synthase-coupling factor 6, mitochondrial antibody
see all
Images
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Western blot - Anti-ATP5J antibody (ab65841)Anti-ATP5J antibody (ab65841) at 1 µg/ml + Rat liver tissue lysate
Predicted band size: 13 kDa
Observed band size: 18 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5J antibody (ab65841)ab65841 at 2µg/ml staining ATP5J in Mammary cancer by Immunohistochemistry, Paraffin-embedded tissue (DAB chromogenic reaction).
References
This product has been referenced in:
- Zeng Z et al. Cyclic mechanical stretch promotes energy metabolism in osteoblast-like cells through an mTOR signaling-associated mechanism. Int J Mol Med 36:947-56 (2015). Read more (PubMed: 26251974) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"