Overview

  • Product name
  • Description
    Rabbit polyclonal to ATP5L
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within N terminal amino acids 1-53 of Human ATP5L (O75964).

  • Positive control
    • HepG2 whole cell lysate; Human Cal27 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab126181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 11 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.
  • Sequence similarities
    Belongs to the ATPase g subunit family.
  • Cellular localization
    Mitochondrion. Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP synthase subunit g antibody
    • ATP synthase, H+ transporting, mitochondrial F1F0, subunit g antibody
    • ATP synthase, H+ transporting, mitochondrial Fo complex, subunit G antibody
    • ATP5L antibody
    • ATP5L_HUMAN antibody
    • ATPase subunit g antibody
    • F1Fo ATP synthase complex Fo membrane domain g subunit antibody
    • mitochondrial antibody
    see all

Images

  • Anti-ATP5L antibody (ab126181) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 11 kDa
  • ab126181, at 1/500 dilution, staining ATP5L in paraffin-embedded Human Cal27 xenograft tissue by Immunohistochemistry.

References

This product has been referenced in:
  • Sakurai T  et al. RNA-binding motif protein 47 inhibits Nrf2 activity to suppress tumor growth in lung adenocarcinoma. Oncogene 35:5000-9 (2016). Read more (PubMed: 26923328) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab126181.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up