Overview

  • Product name
  • Description
    Rabbit polyclonal to ATP5L
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 2-66 of Human ATP5L.

  • Positive control
    • Human stomach tissue; Rt4, U 251 MG and Human liver lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab150920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/250. Predicted molecular weight: 11 kDa.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.
  • Sequence similarities
    Belongs to the ATPase g subunit family.
  • Cellular localization
    Mitochondrion. Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP synthase subunit g antibody
    • ATP synthase, H+ transporting, mitochondrial F1F0, subunit g antibody
    • ATP synthase, H+ transporting, mitochondrial Fo complex, subunit G antibody
    • ATP5L antibody
    • ATP5L_HUMAN antibody
    • ATPase subunit g antibody
    • F1Fo ATP synthase complex Fo membrane domain g subunit antibody
    • mitochondrial antibody
    see all

Images

  • All lanes : Anti-ATP5L antibody (ab150920) at 1/100 dilution

    Lane 1 : RT4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Predicted band size: 11 kDa

  • Immunohistochemical analysis of Paraffin-embedded Human stomach tissue labeling ATP5L with ab150920 at 1/50 dilution.

References

ab150920 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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