Recombinant
RabMAb

Anti-ATP5L2 + ATP5L [EPR15636] antibody (ab191417)

Overview

  • Product name
    Anti-ATP5L2 + ATP5L [EPR15636] antibody
  • Description
    Rabbit monoclonal [EPR15636] to ATP5L2 + ATP5L
  • Tested applications
    Suitable for: ICC/IF, IP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human ATP5L2 + ATP5L aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q7Z4Y8

  • Positive control
    • HeLa, Human fetal liver and Human pancreas lysates; Human kidney tissue; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab191417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
IP 1/100.
WB 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Relevance
    Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain. Minor subunit located with subunit a in the membrane.
  • Cellular localization
    Mitochondrial
  • Database links
  • Alternative names
    • ATP synthase subunit g 2, mitochondrial antibody
    • ATP synthase subunit g, mitochondrial antibody
    • ATP5K2 antibody
    • ATP5L antibody
    • ATP5L2 antibody
    • ATPase subunit g 2 antibody
    • ATPase subunit g antibody
    see all

Images

  • Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling ATP5L2 + ATP5L with ab191417 at 1/100 dilution (19μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).

    Negative controls: anti-ATP5L2 at 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution.

     

  • All lanes : Anti-ATP5L2 + ATP5L [EPR15636] antibody (ab191417) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Human fetal liver tissue lysate
    Lane 3 : Human pancreas tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 11 kDa
  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ATP5L2 + ATP5L with ab191417 at 1/100 dilution (19.4 μg/ml)  followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
    Inset: Negative control: using PBS instead of primary antibody.

  • Western blot analysis of immunoprecipitation pellet from Human fetal liver lysate immunoprecipitated using ab191417 at 1/100 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

     

References

ab191417 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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