Overview

  • Product name
    Anti-ATP5O antibody [4C11C10D12]
    See all ATP5O primary antibodies
  • Description
    Mouse monoclonal [4C11C10D12] to ATP5O
  • Specificity
    Antigen retrieval is required for ICC. Immediately before the permeabilization step, heat the coverslips for 10 minutes in 100mM Tris, 5% urea, pH 9.5 at 95°C for 10 minutes.
  • Tested applications
    Suitable for: IHC-Fr, ICC/IF, WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Cow, Human, African green monkey
    Does not react with: Mouse, Rat
  • Immunogen

    Recombinant mature Human ATP5O.

  • Positive control
    • Isolated mitochondria from Human heart and Bovine heart, Cultured Human embryonic lung-derived fibroblasts (strain MRC5), Human colon tissue, HL-60 cells
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

Our Abpromise guarantee covers the use of ab110276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 25223649
ICC/IF Use a concentration of 0.5 - 1 µg/ml.

Perform heat-induced antigen-retrieval with 100mM Tris, 5% urea, pH 9.5 at 95°C for 10 minutes immediately before permeabilization.

WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 23 kDa.
Flow Cyt Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P 1/500.

Target

  • Function
    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
  • Sequence similarities
    Belongs to the ATPase delta chain family.
  • Cellular localization
    Mitochondrion. Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP synthase O subunit mitochondrial precursor antibody
    • ATP synthase subunit O antibody
    • ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit antibody
    • ATP5O antibody
    • ATPO antibody
    • ATPO_HUMAN antibody
    • mitochondrial antibody
    • Mitochondrial ATP synthase, O subunit antibody
    • Oligomycin sensitivity conferral protein antibody
    • OSCP antibody
    see all

Images

  • All lanes : Anti-ATP5O antibody [4C11C10D12] (ab110276) at 0.5 µg/ml

    Lane 1 : Human heart mitochondria at 5 µg
    Lane 2 : Bovine heart mitochondria at 1 µg


    Predicted band size : 23 kDa
  • Human colon tissue fixed with 4% PFA and antibody detection with ab110276 using the ABC system. ab110276 diluted 1/500 and incubated for 1 hour. Sections were incubated in peroxidase-conjugated rabbit anti-mouse immunoglobulins (diluted 1/100 in 4% BSA in PBST) for 1 hour at room temp.
  • Mitochondrial localization of ATP5O. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110276 (0.5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/ml).
  • HL-60 cells were stained with 1 µg/mL ab110276 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • All lanes : Anti-ATP5O antibody [4C11C10D12] (ab110276) at 1 µg/ml

    Lane 1 : HDFn (human) cell lysates at 20 µg
    Lane 2 : COS7 (monkey) cell lysates at 20 µg
    Lane 3 : H4IIE (rat) cell lysates at 20 µg
    Lane 4 : MEF (mouse) cell lysates at 20 µg
    Lane 5 : bovine heart mitochondria lysates at 5 µg


    Predicted band size : 23 kDa

    Secondary antibody: Goat-Anti-Mouse-IR800 1:4000

References

This product has been referenced in:
  • Anand R  et al. Mic13 Is Essential for Formation of Crista Junctions in Mammalian Cells. PLoS One 11:e0160258 (2016). WB . Read more (PubMed: 27479602) »
  • Hiniker A  et al. Axial mitochondrial myopathy in a patient with rapidly progressive adult-onset scoliosis. Acta Neuropathol Commun 2:137 (2014). IHC-Fr ; Human . Read more (PubMed: 25223649) »

See all 13 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Bovine papillomavirus Tissue lysate - whole (heart)
Total protein in input
5 µg
Immuno-precipitation step
Protein A/G
Specification
heart
Username

Xiang Yin

Verified customer

Submitted Jun 19 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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