Overview

  • Product name
  • Description
    Rabbit polyclonal to ATP5O
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Monkey
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 18-211 of Human ATP5O (Uniprot P48047).

  • Positive control
    • 293T, HeLa, HepG2, Molt4 and Raji whole cell lysates, CL1-0 xenograft

Properties

Applications

Our Abpromise guarantee covers the use of ab154232 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 23 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)

Target

  • Function
    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
  • Sequence similarities
    Belongs to the ATPase delta chain family.
  • Cellular localization
    Mitochondrion. Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP synthase O subunit mitochondrial precursor antibody
    • ATP synthase subunit O antibody
    • ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit antibody
    • ATP5O antibody
    • ATPO antibody
    • ATPO_HUMAN antibody
    • mitochondrial antibody
    • Mitochondrial ATP synthase, O subunit antibody
    • Oligomycin sensitivity conferral protein antibody
    • OSCP antibody
    see all

Images

  • Anti-ATP5O antibody (ab154232) at 1/1000 dilution + HepG2 whole cell
    lysate at 30 µg

    Predicted band size: 23 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded CL1-0 xenograft labeling ATP5O with ab154232 antibody at 1/100.

References

ab154232 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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