Overview

  • Product name
  • Description
    Rabbit polyclonal to ATP6AP1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 251 - 300 (SPVIHPPVSY NDTAPRILFW AQNFSVAYKD QWEDLTPLTF GVQELNLTGS) of Human ATP6AP1 (NP_001174).

  • Positive control
    • OVCAR-3 cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab104339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 52 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance
    ATP6AP1 is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme, which is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. V-ATPase dependent organelle acidification is necessary for intracellular processes such as receptor-mediated endocytosis, protein sorting and zymogen activation.
  • Cellular localization
    Vacuole membrane. Single-pass membrane protein.
  • Database links
  • Alternative names
    • 16A antibody
    • Ac45 antibody
    • ATP6IP1 antibody
    • ATP6S1 antibody
    • ATPase H+ transporting lysosomal (vacuolar proton pump) subunit 1 antibody
    • ATPase H+ transporting lysosomal accessory protein 1 antibody
    • ATPase H+ transporting lysosomal interacting protein 1 antibody
    • CF2 antibody
    • H ATPase subunit antibody
    • MGC129781 antibody
    • ORF antibody
    • Protein XAP 3 antibody
    • Protein XAP3 antibody
    • V ATPase Ac45 subunit antibody
    • V ATPase S1 accessory protein antibody
    • V ATPase subunit S1 antibody
    • V type proton ATPase subunit S1 antibody
    • Vacuolar ATP synthase subunit S1 antibody
    • Vacuolar proton pump subunit S1 antibody
    • VATPS1 antibody
    • XAP 3 antibody
    • XAP3 antibody
    see all

Anti-ATP6AP1 antibody images

  • Anti-ATP6AP1 antibody (ab104339) at 1 µg/ml + OVCAR-3 cell lysate at 10 µg

    Predicted band size : 52 kDa

References for Anti-ATP6AP1 antibody (ab104339)

ab104339 has not yet been referenced specifically in any publications.

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Immunohistochemistry on frozen sections is the best technique for snap frozen sections. Please click the link below to know about IHC-FR

http://www.abcam.com/index.html?pageconfig=resource&rid=11383...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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