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Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human ATP6IP2.
(Peptide available as ab41522.)
Our Abpromise guarantee covers the use of ab40790 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 39 kDa).|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 21628356ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|IHC-P||Use at an assay dependent concentration. PubMed: 23077099|
|IHC-FoFr||1/100. See Abreview.|
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use a concentration of 5 µg/ml.|
ab40790 staining ATP6IP2 in rat kidney tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/100 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG was used at 1/1000 dilution, as secondary.
This image is courtesy of an Abreview submitted by Dr Sophie Pezet.