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Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human ATP6IP2.
(Peptide available as ab41522.)
Our Abpromise guarantee covers the use of ab40790 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 39 kDa).|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 21628356
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||Use at an assay dependent concentration. PubMed: 23077099|
|IHC-FoFr||1/100. See Abreview.|
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use a concentration of 5 µg/ml.|
ab40790 staining ATP6IP2 in rat kidney tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat. The primary antibody was diluted, 1/100 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG was used at 1/1000 dilution, as secondary.
This image is courtesy of an Abreview submitted by Dr Sophie Pezet.
ab40790 staining ATP6IP2 in Human thoraci aortic sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone, permeabilized with Novacastra kit and blocked with Novacastra kit blocker for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. An undiluted HRP-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody
ATP6IP2 was immunoprecipitated using 0.5 mg rat kidney tissue lysate, 5 µg of Rabbit polyclonal to ATP6IP2 and 50 µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10 minutes, rat kidney tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10 minutes under agitation.
Proteins were eluted by addition of 40 µl SDS loading buffer and incubated for 10 minutes at 70oC; 10 µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab40790.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 42 kDa; ATP6IP2