Overview

  • Product nameAnti-ATP6IP2 antibody
    See all ATP6IP2 primary antibodies
  • Description
    Rabbit polyclonal to ATP6IP2
  • Tested applicationsSuitable for: WB, Flow Cyt, IHC-P, IHC-FoFr, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Chicken, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human ATP6IP2.

    (Peptide available as ab41522.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Human Brain; Human Testis; Human Liver; Human Skeletal Muscle

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab40790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 39 kDa).
Flow Cyt Use at an assay dependent concentration. PubMed: 21628356ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration. PubMed: 23077099
IHC-FoFr 1/100. See Abreview.
IHC-Fr Use at an assay dependent concentration.
IP Use a concentration of 5 µg/ml.

Target

  • FunctionFunctions as a renin and prorenin cellular receptor. May mediate renin-dependent cellular responses by activating ERK1 and ERK2. By increasing the catalytic efficiency of renin in AGT/angiotensinogen conversion to angiotensin I, it may also play a role in the renin-angiotensin system (RAS).
  • Tissue specificityExpressed in brain, heart, placenta, liver, kidney and pancreas. Barely detectable in lung and skeletal muscles. In the kidney cortex it is restricted to the mesangium of glomeruli. In the coronary and kidney artery it is expressed in the subendothelium, associated to smooth muscles where it colocalizes with REN. Expressed in vascular structures and by syncytiotrophoblast cells in the mature fetal placenta.
  • Involvement in diseaseDefects in ATP6AP2 are a cause of mental retardation X-linked with epilepsy (MRXE) [MIM:300423]. MRXE is a syndromic mental retardation. Patients manifest mild to moderate mental retardation associated with epilepsy, delays in motor milestones and speech acquisition in infancy.
  • Post-translational
    modifications
    Phosphorylated.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • APT6M8 9 antibody
    • APT6M8-9 antibody
    • ATP6AP2 antibody
    • ATP6IP2 antibody
    • ATP6M8-9 antibody
    • ATPase H(+)-transporting lysosomal accessory protein 2 antibody
    • ATPase H(+)-transporting lysosomal-interacting protein 2 antibody
    • ATPase H+ transporting lysosomal accessory protein 2 antibody
    • ATPase H+ transporting lysosomal interacting protein 2 antibody
    • ATPase H+ transporting lysosomal vacuolar proton pump membrane sector associated protein M8 9 antibody
    • ATPase membrane sector associated protein M8 9 antibody
    • ATPase, H+ transporting, lysosomal (vacuolar proton pump) membrane sector associated protein M8 9 antibody
    • CAPER antibody
    • ELDF10 antibody
    • Embryonic liver differentiation factor 10 antibody
    • ER localized type I transmembrane adaptor antibody
    • ER-localized type I transmembrane adaptor antibody
    • HT028 antibody
    • M8 9 antibody
    • M8-9 antibody
    • MGC99577 antibody
    • MRXE antibody
    • MSTP009 antibody
    • N14F antibody
    • Renin receptor antibody
    • Renin/prorenin receptor antibody
    • RENR_HUMAN antibody
    • V ATPase M8 9 subunit antibody
    • V ATPase M8.9 subunit antibody
    • V-ATPase M8.9 subunit antibody
    • Vacuolar ATP synthase membrane sector associated protein M8 9 antibody
    • Vacuolar ATP synthase membrane sector-associated protein M8-9 antibody
    • vacuolar proton ATP synthase membrane sector associated protein M8 9 antibody
    • XMRE antibody
    see all

Anti-ATP6IP2 antibody images

  • All lanes : Anti-ATP6IP2 antibody (ab40790) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human testis tissue lysate - total protein (ab30257)
    Lane 3 : Human liver tissue lysate - total protein (ab29889)
    Lane 4 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 39 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • ATP6IP2 was immunoprecipitated using 0.5mg Rat Kidney tissue lysate, 5µg of Rabbit polyclonal to ATP6IP2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Rat Kidney tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab40790.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 42kDa; ATP6IP2
  • ab40790 staining ATP6IP2 in rat kidney tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/100 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG was used at 1/1000 dilution, as secondary.

    This image is courtesy of an Abreview submitted by Dr Sophie Pezet.

References for Anti-ATP6IP2 antibody (ab40790)

This product has been referenced in:
  • Nostramo R  et al. Regulation of nonclassical renin-angiotensin system receptor gene expression in the adrenal medulla by acute and repeated immobilization stress. Am J Physiol Regul Integr Comp Physiol 308:R517-29 (2015). IHC-P . Read more (PubMed: 25589013) »
  • Matavelli LC  et al. In vivo regulation of renal expression of (pro)renin receptor by a low-sodium diet. Am J Physiol Renal Physiol 303:F1652-7 (2012). WB, IHC-P ; Rat . Read more (PubMed: 23077099) »

See all 4 Publications for this product

Product Wall


The immunogen for ab40790 is C-terminal, thus it should be able to detect both the soluble and full length form of ATP6IP2. However, the 2 forms only have a difference of 1.5 kDa in MW, which is very hard to distinguish via WB. If a low primary an...

Read More

Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and it is regrettable th...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (human thoraci aortic section- fresh frozen)
Specification human thoraci aortic section- fresh frozen
Fixative Acetone
Permeabilization Yes - NOVACASTRA KIT COMPONENT
Blocking step (agent) for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 16 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Kidney sections)
Specification Kidney sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

Submitted Jun 05 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"