Overview

  • Product nameAnti-ATP6IP2 antibody
    See all ATP6IP2 primary antibodies
  • Description
    Rabbit polyclonal to ATP6IP2
  • SpecificityDetects endogenous levels of total ATP6IP2 protein.
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (Human) from an internal region

  • Positive control
    • Human brain tissue COLO205 and HeLa cell extracts HeLa cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab64957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.
ELISA 1/20000.

Target

  • FunctionFunctions as a renin and prorenin cellular receptor. May mediate renin-dependent cellular responses by activating ERK1 and ERK2. By increasing the catalytic efficiency of renin in AGT/angiotensinogen conversion to angiotensin I, it may also play a role in the renin-angiotensin system (RAS).
  • Tissue specificityExpressed in brain, heart, placenta, liver, kidney and pancreas. Barely detectable in lung and skeletal muscles. In the kidney cortex it is restricted to the mesangium of glomeruli. In the coronary and kidney artery it is expressed in the subendothelium, associated to smooth muscles where it colocalizes with REN. Expressed in vascular structures and by syncytiotrophoblast cells in the mature fetal placenta.
  • Involvement in diseaseDefects in ATP6AP2 are a cause of mental retardation X-linked with epilepsy (MRXE) [MIM:300423]. MRXE is a syndromic mental retardation. Patients manifest mild to moderate mental retardation associated with epilepsy, delays in motor milestones and speech acquisition in infancy.
  • Post-translational
    modifications
    Phosphorylated.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • APT6M8 9 antibody
    • APT6M8-9 antibody
    • ATP6AP2 antibody
    • ATP6IP2 antibody
    • ATP6M8-9 antibody
    • ATPase H(+)-transporting lysosomal accessory protein 2 antibody
    • ATPase H(+)-transporting lysosomal-interacting protein 2 antibody
    • ATPase H+ transporting lysosomal accessory protein 2 antibody
    • ATPase H+ transporting lysosomal interacting protein 2 antibody
    • ATPase H+ transporting lysosomal vacuolar proton pump membrane sector associated protein M8 9 antibody
    • ATPase membrane sector associated protein M8 9 antibody
    • ATPase, H+ transporting, lysosomal (vacuolar proton pump) membrane sector associated protein M8 9 antibody
    • CAPER antibody
    • ELDF10 antibody
    • Embryonic liver differentiation factor 10 antibody
    • ER localized type I transmembrane adaptor antibody
    • ER-localized type I transmembrane adaptor antibody
    • HT028 antibody
    • M8 9 antibody
    • M8-9 antibody
    • MGC99577 antibody
    • MRXE antibody
    • MSTP009 antibody
    • N14F antibody
    • Renin receptor antibody
    • Renin/prorenin receptor antibody
    • RENR_HUMAN antibody
    • V ATPase M8 9 subunit antibody
    • V ATPase M8.9 subunit antibody
    • V-ATPase M8.9 subunit antibody
    • Vacuolar ATP synthase membrane sector associated protein M8 9 antibody
    • Vacuolar ATP synthase membrane sector-associated protein M8-9 antibody
    • vacuolar proton ATP synthase membrane sector associated protein M8 9 antibody
    • XMRE antibody
    see all

Anti-ATP6IP2 antibody images

  • Immunohistochemical analysis of paraffin-embedded human brain tissue using ab64957 at 1/50 dilution. Right hand panel was also treated with immunising peptide.
  • All lanes : Anti-ATP6IP2 antibody (ab64957) at 1/500 dilution

    Lane 1 : COLO205 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : HeLa cell extract with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 39 kDa
    Observed band size : 39 kDa
  • Immunofluorescence analysis of HeLa cells, using ab64957 at 1/500 dilution. Right hand panel was also treated with immunising peptide.

References for Anti-ATP6IP2 antibody (ab64957)

This product has been referenced in:
  • Alzayadneh EM & Chappell MC Nuclear expression of renin-angiotensin system components in NRK-52E renal epithelial cells. J Renin Angiotensin Aldosterone Syst N/A:N/A (2014). ICC/IF . Read more (PubMed: 24961503) »
  • White AJ  et al. Expression of classical components of the renin-angiotensin system in the human eye. J Renin Angiotensin Aldosterone Syst N/A:N/A (2014). IHC ; Human . Read more (PubMed: 25287897) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate, pH6
Permeabilization No
Specification Brain
Blocking step Dako dual enzyme block, Dako protein block as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 21°C
Fixative Formaldehyde
Username

Dr. Jean-Martin Lapointe

Verified customer

Submitted Apr 21 2015

Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and it is regrettable th...

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Thank you for your message.

I am sincerely sorry, I have gone back through the details and realize that I originally made a mistake regarding tested species. It is mouse, chicken and cow that this antibody isonly predicted for. The antibody ...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided willgive us with vital information...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"